Evaluation of the sulfate-reducing bacterial population associated with stored swine slurry
Evaluation of the sulfate-reducing bacterial population associated with stored swine slurry
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DOI：
10.1016/j.anaerobe.2008.03.003
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发表时间：
2008-06-01
期刊：
影响因子：
2.3
通讯作者：
Cotta, Michael A.
中科院分区：
文献类型：
--
作者：
Cook, Kimberly L.;Whitehead, Terence R.;Cotta, Michael A.
硫酸盐还原菌 (SRB) 产生的硫化氢是厌氧猪粪储存系统排放的最强烈的恶臭之一。然而，人们对这些系统中SRB的流行程度和多样性知之甚少。本研究的目的是评估猪粪储存系统中的 SRB 种群，并开发针对四个 SRB 群体的定量实时 PCR (QRT-PCR) 检测方法。异化亚硫酸还原酶 (DSR) 基因序列是从储存在地下坑（43 个克隆）或泻湖（34 个克隆）中的猪浆中获得的。 QRT-PCR 检测旨在针对四个新 SRB 组的 dsrA 基因。来自浆液样品的 dsrA 克隆序列与来自三种不同培养的 SRB（Desulfobulbus sp.）的序列分组。 （46 个克隆），Desulfovibrio sp. （24 个克隆和 5 个分离株）和脱硫杆菌属 sp。 （7 个克隆）。然而，来自猪浆液克隆的 DsrA 序列与培养生物的相似度通常低于 85%。在地下废物储存坑中检测到了所有四个目标SRB组的SRB（6.6 x 10（3）-8.5 x 10（7）dsrA拷贝mL（-1）浆料），而在泻湖中仅检测到两组SRB（3.2 x 10（5）-2.5 x 10（6）dsrA拷贝mL（-1）浆料）。迄今为止，这是唯一评估任何牲畜废物储存系统中SRB的系统发育和浓度的研究。新的 QRT-PCR 检测应有助于对牲畜废物储存系统中四类新型 SRB 进行灵敏、特异的检测。由爱思唯尔有限公司出版
Hydrogen sulfide, produced by sulfate-reducing bacteria (SRB), is one of the most potent malodors emitted from anaerobic swine waste storage systems. However, little is known about the prevalence and diversity of SRB in those systems. The goals of this study were to evaluate the SRB population in swine manure storage systems and to develop quantitative, real-time PCR (QRT-PCR) assays to target four of the SRB groups. Dissimilatory sulfite reductase (DSR) gene sequences were obtained from swine slurry stored in underground pits (43 clones) or in lagoons (34 clones). QRT-PCR assays were designed to target the dsrA gene of four novel groups of SRB. Sequences of dsrA clones from slurry samples grouped with those from three different cultured SRB: Desulfobulbus sp. (46 clones), Desulfovibrio sp. (24 clones and 5 isolates), and Desulfobacterium sp. (7 clones). However, DsrA sequences from swine slurry clones were generally less than 85% similar to those of cultured organisms. SRB from all four targeted SRB groups were detected in underground waste storage pits (6.6 x 10(3) -8.5 x 10(7) dsrA copies mL(-1) slurry), while only two groups of SRB were detected in lagoons (3.2 x 10(5) -2.5 x 10(6) dsrA copies mL(-1) slurry). To date, this is the only study to evaluate the phylogeny and concentration of SRB in any livestock waste storage system. The new QRT-PCR assays should facilitate sensitive, specific detection of the four novel groups of SRB in livestock waste Storage systems. Published by Elsevier Ltd.