Sleep Deprivation Potentiates HPA Axis Stress Reactivity in Healthy Adults
Sleep Deprivation Potentiates HPA Axis Stress Reactivity in Healthy Adults
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DOI：
10.1037/a0034219
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发表时间：
2014-11-01
影响因子：
4.2
通讯作者：
Dinges, David
中科院分区：
文献类型：
--
作者：
Minkel, Jared;Moreta, Marisa;Dinges, David
目的：本文描述了一项旨在研究睡眠剥夺对健康成年人生理应激反应的影响的实验。方法：26名年龄在22-49岁之间的参与者完成了一项为期3晚的实验室实验，随机分为一晚睡眠剥夺或正常睡眠控制条件。经过一夜的基线睡眠后，12名参与者被剥夺了睡眠，14名没有。在睡眠操作之后，每个参与者都完成了特里尔社会压力测试，这项任务需要在一个严厉的三人小组面前发表演讲并进行困难的算术。压力源是从下午5点开始施加的-下午5：30，并在应激前20和5分钟（基线）以及应激后5、20和40分钟收集唾液样本。对样本进行了皮质醇（HPA轴的生物标志物）和α-淀粉酶（交感-肾上腺髓质系统的推定生物标志物）检测。结果如下：睡眠剥夺与基线时较高的皮质醇水平（p <0.0001）以及相对于对照参与者对压力源的放大皮质醇反应相关（p（交互作用）= 0.0039）。α-淀粉酶显示压力源的显著主要影响（p = 0.0026），但在基线或对压力源的反应中，睡眠丧失没有影响。结论：睡眠剥夺与静息皮质醇释放升高和对应激源的过度皮质醇反应相关，这表明健康成人的HPA轴反应升高。这种反应的幅度的个体差异可能代表与皮质醇暴露增加相关的心理和身体健康后果的风险因素。
Objective: This article describes an experiment that was designed to investigate the effects of sleep deprivation on physiological stress responses in healthy adults. Method: Twenty-six participants, ages 22-49, completed a 3-night laboratory experiment with randomization to one night of sleep-deprivation or a normal-sleep control condition. After a night of baseline sleep, 12 participants were sleep deprived and 14 were not. After the sleep manipulation, each participant completed the Trier Social Stress Test, a task that requires delivering a speech and performing difficult arithmetic in front of a stern, three-person panel. The stressor was administered from 5:00 p.m.- 5:30 p.m. and saliva samples were collected 20 and 5 min before (baseline) and 5, 20, and 40 min after the stressor. Samples were assayed for cortisol (a biomarker for the HPA axis) and alpha-amylase (a putative biomarker for the sympatho-adrenal medullar system). Results: Sleep deprivation was associated with higher cortisol levels at baseline (p < .0001) and an amplified cortisol response to the stressor relative to control participants (p(interaction) = 0.0039). Alpha-amylase showed a significant main effect of the stressor (p = .0026), but there was no effect of sleep loss at baseline or in response to the stressor. Conclusions: Sleep deprivation is associated with both elevated resting cortisol release and with an exaggerated cortisol response to a stressor indicative of elevated HPA axis responses in healthy adults. Individual differences in the magnitude of this response may represent a risk factor for psychological and physical health consequences associated with heightened cortisol exposure.