Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors
Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors
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DOI：
10.1073/pnas.0837064100
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发表时间：
2003-04-29
影响因子：
11.1
通讯作者：
Dai, HJ
中科院分区：
文献类型：
--
作者：
Chen, RJ;Bangsaruntip, S;Dai, HJ
用于生物测定应用的新型纳米材料代表了纳米技术和纳米生物技术的快速发展领域。在这里，我们对单壁碳纳米管作为研究表面蛋白质和蛋白质-蛋白质结合以及开发高度特异性电子生物分子探测器的平台进行了探索。纳米管上的非特异性结合是一种在多种蛋白质中发现的现象，通过固定聚环氧乙烷链可以克服这种现象。然后提出了一种通用方法，通过将靶蛋白的特异性受体与聚环氧乙烷功能化的纳米管缀合来选择性识别和结合靶蛋白。该方案与纳米管电子器件的灵敏度相结合，使得高度特异性的电子传感器能够用于检测临床上重要的生物分子，例如与人类自身免疫性疾病相关的抗体。
Novel nanomaterials for bioassay applications represent a rapidly progressing field of nanotechnology and nanobiotechnology. Here, we present an exploration of single-walled carbon nanotubes as a platform for investigating surface-protein and protein-protein binding and developing highly specific electronic biomolecule detectors. Nonspecific binding on nanotubes, a phenomenon found with a wide range of proteins, is overcome by immobilization of polyethylene oxide chains. A general approach is then advanced to enable the selective recognition and binding of target proteins by conjugation of their specific receptors to polyethylene oxide-functionalized nanotubes. This scheme, combined with the sensitivity of nanotube electronic devices, enables highly specific electronic sensors for detecting clinically important biomolecules such as antibodies associated with human autoimmune diseases.