Immunocytochemical evidence for phorbol ester-induced protein kinase C translocation in HL60 cells.
Immunocytochemical evidence for phorbol ester-induced protein kinase C translocation in HL60 cells.
复制标题
HL60 细胞中佛波酯诱导蛋白激酶 C 易位的免疫细胞化学证据。
DOI：
10.1016/0006-291x(86)91047-8
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发表时间：
1986
影响因子：
3.1
通讯作者：
Kuo,JF
中科院分区：
文献类型：
--
作者：
Shoji,M;Girard,PR;Mazzei,GJ;Vogler,WR;Kuo,JF
The ability of tumor promoting 12-O-tetradecanoylphorbol-13-acetate (TPA) to redistribute protein kinase C in human promyelocytic leukemic HL60 cells was investigated. It was found that TPA caused a rapid translocation (within 10 min) of protein kinase C from the cytosolic (soluble) fraction to the particulate (membrane) fraction, as determined indirectly by assaying for the enzyme activity or by immunoblotting of the enzyme protein in the isolated subcellular fractions. Immunocytochemical localization of the enzyme demonstrated directly that the TPA caused an enzyme translocation to the plasma membrane. These findings suggest that translocation to the plasma membrane of the enzyme may represent initial events related to the TPA effect on terminal differentiation of HL60 cells to monocytes/macrophages.
