Optimized bone regeneration based on sustained release from three-dimensional fibrous PLGA/HAp composite scaffolds loaded with BMP-2
Optimized bone regeneration based on sustained release from three-dimensional fibrous PLGA/HAp composite scaffolds loaded with BMP-2
复制标题
DOI：
10.1002/bit.21648
复制
发表时间：
2008-03-01
影响因子：
3.8
通讯作者：
Wang, Chi-Hwa
中科院分区：
文献类型：
--
作者：
Fu, Yin-Chih;Nie, Hemin;Wang, Chi-Hwa
严重骨缺损的现代治疗仍然是骨科手术领域的一个重大挑战。结合生长因子的工程生物材料已成为骨修复和再生的新治疗选择。我们的方法是将骨形态发生蛋白-2（BMP-2）以不同的方式封装到聚合物基质中，并在裸鼠模型中表征其各自的性能。本研究的主要目的是观察通过静电纺丝法制备的负载BMP-2的PLGA/HAp复合纤维支架是否具有促进骨再生的作用。假设不同的BMP-2负载方法和不同的HAp含量可以改变BMP-2在体内的释放曲线，从而改变支架在骨再生中的性能。首先，研究了支架的机械强度和HAp纳米粒子在支架中的分布。第二，通过6周的裸鼠体内实验，检测支架材料的体内性能，通过血清BMP-2浓度、ALP活性、X线定性、H&E/IHC组织染色等检测手段，监测新骨的生长情况及相应生化指标的变化。结果表明，所制备的PLGA/HAp复合支架具有良好的形态和力学性能，HAp纳米粒子均匀分散在PLGA基质中。动物实验结果表明，纤维状PLGA/HAp复合支架释放的BMP-2生物活性保持良好，进一步促进了体内新骨的形成和节段性缺损的愈合。结论：BMP-2负载的PLGA/HAp复合支架具有良好的骨愈合性能。
Contemporary treatment of critical bone defect remains a significant challenge in the field of orthopedic surgery. Engineered biomaterials combined with growth factors have emerged as a new treatment alternative in bone repair and regeneration. Our approach is to encapsulate bone morphogenetic protein-2 (BMP-2) into a polymeric matrix in different ways and characterize their individual performance in a nude mouse model. The main objective of this study is to examine whether the PLGA/HAp composite fibrous scaffolds loaded with BMP-2 through electrospinning can improve bone regeneration. The hypothesis is that different loading methods of BMP-2 and different HAp contents in scaffolds can alternate the release profiles of BMP-2 in vivo, therefore modify the performance of scaffolds in bone regeneration. Firstly, mechanical strength of scaffolds and HAp nanoparticles distribution in scaffolds were investigated. Secondly, nude mice experiments extended to 6 weeks were carried out to test the in vivo performance of these scaffolds, in which measurements, like serum BMP-2 concentration, ALP activity, X-ray qualification, and H&E/IHC tissue staining were utilized to monitor the growth of new bone and the changes of the corresponding biochemical parameters. The results showed that the PLGA/HAp composite scaffolds developed in this study exhibited good morphology/mechanical strength and HAp nanoparticles were homogeneously dispersed inside PLGA matrix. Results from the animal experiments indicate that the bioactivity of BMP-2 released from the fibrous PLGA/HAp composite scaffolds is well maintained, which further improves the formation of new bone and the healing of segmental defects in vivo. It is concluded that BMP-2 loaded PLGA/HAp composite scaffolds are promising for bone healing.