Panax notoginseng saponins attenuate atherosclerosis in rats by regulating the blood lipid profile and an anti-inflammatory action
Panax notoginseng saponins attenuate atherosclerosis in rats by regulating the blood lipid profile and an anti-inflammatory action
复制标题
三七皂苷通过调节血脂和抗炎作用减轻大鼠动脉粥样硬化
DOI：
10.1111/j.1440-1681.2008.04997.x
复制
发表时间：
2008-10-01
影响因子：
2.9
通讯作者：
Tang, Zhong
中科院分区：
文献类型：
--
作者：
Zhang, Yi-Guan;Zhang, Hai-Gang;Tang, Zhong
1.三七总皂苷（Panax notoginseng saponins，PNS）的抗动脉粥样硬化作用已有研究报道。本研究的目的是探讨三七总皂苷抗动脉粥样硬化作用的分子机制及其与炎症反应的关系.将30只大鼠随机分为3组，即对照组、酵母多糖A致炎组（Zym组）和PNS治疗组。三组大鼠腹腔注射液体石蜡，酵母聚糖A（20 mg/kg，i. p.，每3天一次）或酵母聚糖A和PNS（100 mg/kg，i. p.，每日一次）。所有动物均喂食高脂肪饲料9周。在预定时间，处死大鼠，收集血液并取出主动脉。采用苏丹Ⅳ染色和透射电镜观察肝组织的病理变化。酶法测定血清脂质。在不同的剪切速率下观察全血粘度。使用GEArray（SuperArray，Frederick，MA，USA）测定心血管疾病特异性基因的表达。Western blotting检测主动脉壁核因子（NF）-κ B/p65及其抑制因子I κ B α的表达水平.在本研究中，与动脉粥样硬化相关的典型病理变化在酵母多糖A诱导的大鼠后，PNS治疗减轻。在PNS治疗组中，血清总胆固醇、甘油三酯和血液粘度显著降低。此外，PNS治疗显著降低了一些炎症因子的基因表达，如整合素、白细胞介素（IL）-18，IL-1 β和基质金属蛋白酶2和9。经PNS治疗后，NF-κ B/p65表达减弱，而I κ B α表达显著增加.总之，PNS通过抗炎作用和调节血脂谱对动脉粥样硬化发挥其治疗作用，并且涉及NF-κ B信号通路。
1. Previous studies have reported on the anti-atherosclerotic effects of Panax notoginseng saponins (PNS). The aim of the present study was to explore the molecular mechanisms responsible for the anti-atherosclerotic effects of PNS and the inflammatory response.2. Thirty rats were randomly divided into three groups, namely a control group, a group, in which zymosan A was used to induce inflammation (Zym group) and a PNS-treated group. Rats in the three groups were administered liquid paraffin (i.p.), zymosan A (20 mg/kg, i.p., once every 3 days) or zymosan A and PNS (100 mg/kg, i.p., once daily), respectively. All animals were fed a high-fat diet for 9 weeks. At scheduled times, rats were killed, blood was collected and the aorta was removed. Pathological changes in aortas were observed using Sudan IV staining and transmission electron microscopy. Serum lipids were measured enzymatically. Whole-blood viscosity was observed at different shear rates. The expression of cardiovascular disease-specific genes was determined using GEArray (SuperArray, Frederick, MA, USA). Western blotting was used to evaluate the expression levels of nuclear factor (NF)-kappa B/p65 and its inhibitor I kappa B alpha in the aortic wall.3. In the present study, typical pathological changes associated with atherosclerosis in rats following induction by zymosan A were alleviated by PNS treatment. In the PNS-treated group, there was a marked reduction in total serum cholesterol, triglycerides and blood viscosity. In addition, PNS treatment significantly decreased the gene expression of some inflammatory factors, such as integrins, interleukin (IL)-18, IL-1 beta and matrix metalloproteinases 2 and 9. The expression of NF-kappa B/p65 was attenuated, whereas the expression Of I kappa B alpha was significantly increased, after treatment with PNS.4. In conclusion, it appears that PNS exerts its therapeutic effects on atherosclerosis through an anti-inflammatory action and regulation of the blood lipid profile and that an NF-kappa B signalling pathway is involved.