Multilocus sequence typing of Candida glabrata reveals geographically enriched clades
Multilocus sequence typing of Candida glabrata reveals geographically enriched clades
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草绿色念珠菌的多焦点序列分型揭示了地理上丰富的支系
DOI：
10.1128/jcm.41.12.5709-5717.2003
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发表时间：
2003-12-01
影响因子：
9.4
通讯作者：
Fox, AJ
中科院分区：
文献类型：
--
作者：
Dodgson, AR;Pujol, C;Fox, AJ
单倍体致病性酵母光滑念珠菌是从血流感染病例中分离的第二种最常见的念珠菌。C. glabrata通过其对氟康唑的敏感性降低而增强。尽管如此，对该物种的流行病学或种群结构知之甚少。我们发展了一种多位点序列分型（MLST）方案。glabrata，并使用它指纹的地理多样性收集的107个临床分离株和2个参考菌株。通过扩增11C的编码区片段并测序来鉴定合适的基因座。glabrata基因在10个无关的分离物。对109株分离株的6个最易变异的基因座（FKS、LEU2、NMT1、TRP1、UGP1和URA3）进行测序。从每个分离物中测序的3，345 bp中，发现81个核苷酸位点是可变的。这些在109株菌株中定义了30种ST。该技术通过与随机扩增多态性DNA和复杂的DNA指纹探针Cg6和Cg12的比较进行了验证。MLST确定了5个主要的分支之间的研究菌株。三个分支表现出显着的地理偏见。我们的数据第一次证明，如此大的地理多样性菌株收集，不同的遗传分支的C。光叶苔在不同的地理区域盛行。
The haploid pathogenic yeast Candida glabrata is the second most common Candida species isolated from cases of bloodstream infection. The clinical relevance of C. glabrata is enhanced by its reduced susceptibility to fluconazole. Despite this, little is known of the epidemiology or population structure of this species. We developed a multilocus sequence typing (MLST) scheme for C. glabrata and used it to fingerprint a geographically diverse collection of 107 clinical isolates and 2 reference strains. Appropriate loci were identified by amplifying and sequencing fragments of the coding regions of 11 C. glabrata genes in 10 unrelated isolates. The 6 most variable loci (FKS, LEU2, NMT1, TRP1, UGP1, and URA3) were sequenced in the collection of 109 isolates. From the 3,345 bp sequenced in each isolate, 81 nucleotide sites were found to be variable. These defined 30 STs among the 109 strains. The technique was validated by comparison with random amplified polymorphic DNA and the complex DNA fingerprinting probes Cg6 and Cg12. MLST identified 5 major clades among the isolates studied. Three of the clades exhibited significant geographical bias. Our data demonstrate for the first time, with such a large geographically diverse strain collection, that distinct genetic clades of C. glabrata prevail in different geographical regions.