Angular dependent light scattering from multicellular spheroids
Angular dependent light scattering from multicellular spheroids
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DOI：
10.1117/1.1427053
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发表时间：
2002-01-01
影响因子：
3.5
通讯作者：
Freyer, JP
中科院分区：
文献类型：
--
作者：
Mourant, JR;Johnson, TM;Freyer, JP
我们证明，细胞与细胞接触和细胞形状变化的影响对哺乳动物成纤维细胞的光散射的角度分布只有很小的影响。这一结果对于光散射作为非侵入性组织诊断（例如癌症检测）的发展非常重要。不伴随内部细胞结构变化的细胞组织变化可能是不可测量的。另一方面，内部细胞结构的变化应该是可测量的，而不会受到整体细胞组织变化的干扰。这项工作的第二个主要结果是，在三维培养系统中生长的致瘤细胞和非致瘤细胞的光散射之间存在微小但显着的差异。光散射差异的原因可能是由于非致瘤细胞停滞在细胞周期的151期，而致瘤细胞继续增殖。 (C) 2002 年光学仪器工程师协会。
We demonstrate that the effects of cell-cell contact and of changes in cell shape have only a minor effect on the angular distribution of light scattering from mammalian fibroblast cells. This result is important for the development of light scattering as a noninvasive too for tissue diagnostics such as cancer detection. Changes in cell organization that are not accompanied by changes in internal cellular structure may riot be measurable. On the other hand, changes in internal cellular structure should be measurable without interference from changes in overall cellular organization. The second major result of this work is that there are small but significant differences between light scattering of tumorigenic and nontumorigenic cells grown in a three-dimensional culture system. The cause of the differences in light Scattering are likely due to the nontumorigenic cells arresting in the 151 phase of the cell cycle, while the turnorigenic cells continue to proliferate. (C) 2002 Society of Photo-optical instrumentation Engineers.