A nucleolar protein RRS1 contributes to chromosome congression
A nucleolar protein RRS1 contributes to chromosome congression
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DOI：
10.1016/j.febslet.2009.05.033
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发表时间：
2009-06-18
期刊：
影响因子：
3.5
通讯作者：
Fukui, Kiichi
中科院分区：
文献类型：
--
作者：
Gambe, Arni E.;Matsunaga, Sachihiro;Fukui, Kiichi
我们在这里报告的功能分析的人核糖体合成调节因子1（RRS 1）蛋白在有丝分裂。我们证明，RRS1定位在核仁间期和有丝分裂期间分布在染色体外周。RNA干扰实验表明，RRS1缺失的细胞显示染色体排列和纺锤体组织异常，导致有丝分裂延迟。RRS1敲除也扰乱了Shugoshin 1的着丝粒定位，并导致姐妹染色单体过早分离。我们的研究结果表明，核仁蛋白RRS1有助于染色体聚集。(C)2009年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
We report here the functional analysis of human Regulator of Ribosome Synthesis 1 (RRS1) protein during mitosis. We demonstrate that RRS1 localizes in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. RNA interference experiments revealed that RRS1-depleted cells show abnormalities in chromosome alignment and spindle organization, which result in mitotic delay. RRS1 knockdown also perturbs the centromeric localization of Shugoshin 1 and results in premature separation of sister chromatids. Our results suggest that a nucleolar protein RRS1 contributes to chromosome congression. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.