Cryptic clonal lineages and genetic diversity in the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) inferred from nuclear and mitochondrial DNA analyses
Cryptic clonal lineages and genetic diversity in the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) inferred from nuclear and mitochondrial DNA analyses
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DOI：
10.1007/s10709-007-9158-1
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发表时间：
2007-06
期刊：
影响因子：
1.5
通讯作者：
K. Morishima;Yuka Nakamura-Shiokawa;E. Bando;Ya-Juan Li;A. Boroń;Md. Mukhlesur Rahman Khan;K. Arai
中科院分区：
文献类型：
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作者：
K. Morishima;Yuka Nakamura-Shiokawa;E. Bando;Ya-Juan Li;A. Boroń;Md. Mukhlesur Rahman Khan;K. Arai
在泥鳅中，已经鉴定出产生未减少的克隆二倍体卵子的无性世系。对来自日本54个地区和中国2个地区的833份标本进行线粒体DNA控制区限制性片段长度多态性分析，筛选出82个隐性克隆的其他家系(S)的候选个体。之所以进行这项分析，是因为三倍体泥土是由于精子核意外地并入克隆人未还原的二倍体卵子而产生的。利用随机扩增多态性DNA(随机扩增多态DNA)-聚合酶链式反应和多位点DNA指纹技术，对新鉴定的3个家系(克隆2-4)和已鉴定的克隆家系(克隆1)的成员进行了分类，并对来自每个克隆家系的两个或多个个体之间的遗传同源性进行了评估。我们通过观察线粒体DNA控制区可变区的核苷酸状态，检测了75种单倍型。由这些序列构建的系统发育树显示出两个高度多样化的分支，A和B，超出了种间遗传分化的常见水平。这一结果表明，我。日本的鳗鱼不是一个单一的物种实体。A分支含有两个克隆特异的mtDNA序列，这些序列可能是这些克隆的母本起源。
In the loachMisgurnus anguillicaudatue, the asexual lineage, which produces unreduced clonal diploid eggs, has been identified. Among 833 specimens collected from 54 localities in Japan and two localities in China, 82 candidates of other lineage(s) of cryptic clones were screened by examining RFLP (restriction fragment length polymorphism)-PCR haplotypes in the control region of mtDNA. This analysis was performed because triploid loaches arise from the accidental incorporation of the sperm nucleus into unreduced diploid eggs of a clone. The categorization of members belonging to three newly identified lineages (clones 2–4) and the previously identified clonal lineage (clone 1) was verified by evaluating the genetic identity between two or more individuals from each clonal lineage based on RAPD (random amplified polymorphic DNA)-PCR and multilocus DNA fingerprints. We detected 75 haplotypes by observing the nucleotide status at variable sites from the control region of mtDNA. Phylogenic trees constructed from such sequences showed two highly diversified clades, A and B, that were beyond the level common for interspecific genetic differentiation. That result suggests thatM. anguillicaudatusin Japan is not a single species entity. Two clone-specific mtDNA sequences were included in clade A, and the loaches with such sequences may be the maternal origin of the clones.