Isoflurane preserves spatial working memory in adult mice after moderate hypoxia
Isoflurane preserves spatial working memory in adult mice after moderate hypoxia
复制标题
DOI：
10.1213/01.ane.0000198637.36539.c1
复制
发表时间：
2006-04-01
影响因子：
5.7
通讯作者：
Blanck, T
中科院分区：
文献类型：
--
作者：
Bekker, A;Shah, R;Blanck, T
围手术期缺氧可能导致术后认知功能障碍。然而，麻醉剂是否会加重或防止缺氧相关的中枢神经系统损伤尚不清楚。我们试图确定是否缺氧单独或与异氟烷的组合破坏小鼠的工作记忆。在这种程度上，我们将成年小鼠分配到四种治疗中的一种，持续1小时：氧气21%，氧气21% +异氟烷1.2%，氧气8%或氧气8% +异氟烷1.2%。在整个实验过程中，小鼠自发呼吸。体温维持在37 ℃ ± 0.5 ℃。允许小鼠恢复24小时以避免残留麻醉剂对神经行为表现的混杂影响。通过使用为小鼠改良的Y迷宫评估工作记忆。对于训练试验，阻断一个臂的入口，并允许小鼠在两个开放臂之间奔跑15分钟，并检查外面的物体。对于1小时后进行的试验性试验，所有的武器都张开了。每只手臂花费的时间由摄像机和相关软件自动记录。在麻醉后1、4和7天测试小鼠。使用不同的手臂作为每次测试的新手臂。使用重复测量方差分析分析性能，然后分析简单主效应，并在适当时使用Newman-Keuls检验进行事后比较。P值< 0.05被认为是显著的。在损伤后1天，经受缺氧（8%氧气持续1小时）的动物在新臂中花费的时间显著更少。然而，这种损害是暂时的。在处理后第四天，低血糖小鼠的表现改善至对照动物的水平。缺氧加异氟烷处理的小鼠未表现出损伤，并且在所有时间点均与对照组相当。缺氧会暂时损害空间记忆任务的表现。看来异氟烷可以防止缺氧的这种有害作用。
Perioperative hypoxia may contribute to postoperative cognitive impairment. It is unknown, however, whether anesthetics exacerbate or protect against hypoxia-related central nervous system impairment. We sought to determine whether hypoxia alone or in combination with isoflurane disrupts working memory in mice. To this extent, we assigned adult mice to one of four treatments for 1 h: oxygen 21%, oxygen 21% + isoflurane 1.2%, oxygen 8%, or oxygen 8% + isoflurane 1.2%. Mice breathed spontaneously throughout the experiment. Body temperature was maintained at 37 degrees C + 0.5 degrees C. Mice were allowed to recover for 24 It to avoid the confounding influence of residual anesthetics on neurobehavioral performance. Working memory was assessed by use of a Y maze modified for mice. For the training trial, entry to one arm was blocked and mice were permitted to run between the two open arms for 15 min and inspect the objects outside. For the test trial, carried out I h later, all arms were open. Time spent in each arm was automatically recorded by a camera and associated software. Mice were tested 1, 4, and 7 days after anesthesia. A different arm was used as the novel arm for each test. Performance was analyzed with repeated-measurements analysis of variance, followed by analysis of simple main effects and by post hoc comparison using Newman-Keuls test when appropriate. P values < 0.05 were considered significant. Animals subjected to hypoxia (8% oxygen for 1 h) spent significantly less time in the novel arm 1 day after the insult. The impairment, however, was transient. Hypoxic mice performance improved to the level of the control animals on the fourth post-treatment day. Mice subjected to hypoxia plus isoflurane exhibited no impairment and were comparable to the control group at all time points. Hypoxia transiently impairs performance in a spatial memory task. It appears that isoflurane protects against this deleterious effect of hypoxia.