Effect of chitosan on a periodontal pathogen Porphyromonas gingivalis
Effect of chitosan on a periodontal pathogen Porphyromonas gingivalis
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DOI：
10.1016/s0378-5173(01)00974-7
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发表时间：
2002-03-20
影响因子：
5.8
通讯作者：
Hincal, AA
中科院分区：
文献类型：
--
作者：
Ikinci, G;Senel, S;Hincal, AA
由于全身给药的缺点，在过去的十年中，用于治疗牙周病的抗菌药物的局部输送系统受到了相当多的关注。理想的制剂应表现出易于递送、在应用部位具有良好的保留性以及药物的受控释放。生物粘附凝胶的应用提供了在口腔中的长时间停留、充分的药物渗透、高功效和可接受性。在牙科和口腔医学领域。壳聚糖是一种生物粘附聚合物，由于其良好的特性，如生物相容性和生物降解性，已被提出了各种应用。本研究的目的是确定壳聚糖制剂（无论是片状还是膜状）对牙周病原体牙龈卟啉单胞菌的抗菌活性。在不存在或存在葡萄糖酸氯己定 (Chx) 的情况下，以 0.1% 和 0.2% 的浓度掺入制剂中，评估了不同分子量和脱乙酰度的壳聚糖的粘度、生物粘附特性和抗菌活性。发现凝胶的流动特性适合局部应用于口腔粘膜和注射到牙周袋中。使用新鲜猪颊粘膜离体检查的凝胶和薄膜的生物粘附性表明，薄膜和凝胶制剂均发挥生物粘附特性并且不受Chx掺入的影响。壳聚糖被证明对牙龈卟啉单胞菌具有抗菌活性，并且高分子量壳聚糖的抗菌活性更高。与单独的 Chx 相比，壳聚糖与 Chx 的组合显示出更高的活性，这将使 Chx 在较低浓度下应用，从而避免其不需要的副作用。壳聚糖薄膜和凝胶凭借其生物粘附特性和抗菌活性，似乎成为牙周病局部治疗的有前景的递送系统。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Local delivery systems of antimicrobial agents for treatment of the periodontal diseases received considerable attention during the past decade due to the disadvantages of the systemic administration. An ideal formulation should exhibit ease of delivery, a good retention at the application site, and a controlled release of the drug. The application of bioadhesive gels provides a long stay in the oral cavity, adequate drug penetration, high efficacy and acceptability. In dentistry and oral medicine. various applications of chitosan, which is a bioadhesive polymer have been proposed due to its favorable properties such as biocompatibility and biodegradability. The aim of this study was to determine the antimicrobial activity of chitosan formulations either in get or film form against a periodontal pathogen, Porphyromonas gingalvalis. The viscosity, bioadhesive properties and antimicrobial activity of chitosans at different molecular weight and deacetylation degree were evaluated in the absence or presence of chlorhexidine gluconate (Chx), incorporated into the formulations at 0.1 and 0.2% concentrations. The flow property of the gels were found to be suitable for topical application on the oral mucosa and to syringe into the periodontal pocket. Bioadhesion of the gels and films examined ex-vivo using fresh porcine buccal mucosa showed that both the film and gel formulations exert bioadhesive properties and was not affected by incorporation of Chx. Chitosan is shown to have an antimicrobial activity against P. gingivalis and this was higher with high molecular weight chitosan. The combination of chitosan with Chx showed a higher activity when compared to that of Chx alone, which would provide Chx application at lower concentrations thus avoiding its unwanted side effects. Chitosan films and gels seem to be promising delivery systems for local therapy of periodontal diseases with its bioadhesive property and antimicrobial activity. (C) 2002 Elsevier Science B.V. All rights reserved.