Identification of gene co-expression networks involved in cold resistance of Lilium lancifolium
Identification of gene co-expression networks involved in cold resistance of Lilium lancifolium
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DOI：
10.1007/s10535-017-0767-y
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发表时间：
2018-06
影响因子：
1.5
通讯作者：
Y. Yong;Wenqi Li;Jingmao Wang;Yue Zhang;Y. M. Lu
中科院分区：
文献类型：
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作者：
Y. Yong;Wenqi Li;Jingmao Wang;Yue Zhang;Y. M. Lu
低温会影响百合的生长发育，限制了商业品种在室外的应用。百合是一种重要的抗寒野生百合，但对其研究较少。金银花在分子水平上耐受冷胁迫。在这项研究中，我们在对照植物和4℃处理1-24小时的植物中鉴定了与冷胁迫相关的基因和转录因子。利用马尔可夫聚类算法构建了一个基于最高倒数等级的基因共表达网络，并将其划分为定义的功能模块，得到了30个基因模块，其中一些基因模块在4℃下的各种胁迫反应中显著丰富。这些基因模块与代谢过程、细胞过程、生物过程的调节、定位的建立和对刺激的反应有关。此外，还发现了三种可能调节下游基因的转录因子，这些基因涉及对刺激的反应。我们进一步研究了这些转录因子的表达模式和组织特异性。本研究中各种感兴趣的基因的功能评估可能会为百合抗寒相关途径成员和调控因子的新发现提供新的线索。
Low temperature can affect the growth and development of lily, limiting the application of commercial cultivars in outdoor.Lilium lancifoliumis an important cold-resistant wild lily, but little is known about howL. lancifoliumtolerates cold stress at the molecular level. In this study, we identified and characterized genes and transcription factors associated with cold stress in control plants and plants treated by 4° C for 1 - 24 h. The construction of a highest reciprocal rank-based gene co-expression network along with its partition into defined functional modules using Markov cluster algorithm resulted in identification of 30 gene modules and some of them were significantly enriched with various kinds of stress response under 4° C. These gene modules were associated with metabolic processes, cellular processes, regulation of biological processes, establishment of localization, and responses to stimuli. Moreover, three transcription factors that may regulate the downstream genes involved in response to stimuli were also found. We further studied the expression pattern and tissue specificity of these transcription factors. The functional evaluation of the various interesting genes in this study will probably provide novel discovery of pathway members and regulators associated with cold resistance in lily.