Automated classification of Plasmodium sporozoite movement patterns reveals a shift towards productive motility during salivary gland infection
Automated classification of Plasmodium sporozoite movement patterns reveals a shift towards productive motility during salivary gland infection
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DOI：
10.1002/biot.200900007
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发表时间：
2009-06-01
影响因子：
4.7
通讯作者：
Frischknecht, Friedrich
中科院分区：
文献类型：
--
作者：
Hegge, Stephan;Kudryashev, Mikhail;Frischknecht, Friedrich
疟疾和其他顶复门寄生虫的侵入阶段使用基于肌动蛋白、肌球蛋白和许多寄生虫特异性蛋白的独特运动机制来侵入宿主细胞和组织。这种运动机制在这些寄生虫生命周期的几个阶段至关重要，使得单个组分成为潜在的药物靶点。疟疾寄生虫的不同阶段表现出惊人的不同运动模式，可能反映了实现成功入侵的不同需求。在这里，我们描述了一个自动子孢子跟踪工具（吐司），它允许快速同时分析几百个运动的疟原虫子孢子，阶段的疟疾寄生虫传播的蚊子。吐司可靠地将子孢子运动的不同模式分类，并且可以用于跟踪运动模式的变化和比较总体运动参数，例如经历某种类型运动的子孢子的平均速度或持久性。这允许比较不同寄生虫种群之间潜在的小差异，并且将使得能够筛选药物文库以发现子孢子运动性的抑制剂。使用吐司，我们发现，孤立的子孢子改变其运动模式，对生产运动在第一周后，蚊子唾液腺感染。
The invasive stages of malaria and other apicomplexan parasites use a unique motility machinery based on actin, myosin and a number of parasite-specific proteins to invade host cells and tissues. The crucial importance of this motility machinery at several stages of the life cycle of these parasites makes the individual components potential drug targets. The different stages of the malaria parasite exhibit strikingly diverse movement patterns, likely reflecting the varied needs to achieve successful invasion. Here, we describe a Tool for Automated Sporozoite Tracking (ToAST) that allows the rapid simultaneous analysis of several hundred motile Plasmodium sporozoites, the stage of the malaria parasite transmitted by the mosquito. ToAST reliably categorizes different modes of sporozoite movement and can be used for both tracking changes in movement patterns and comparing overall movement parameters, such as average speed or the persistence of sporozoites undergoing a certain type of movement. This allows the comparison of potentially small differences between distinct parasite populations and will enable screening of drug libraries to find inhibitors of sporozoite motility. Using ToAST, we find that isolated sporozoites change their movement patterns towards productive motility during the first week after infection of mosquito salivary glands.