Gene-for-gene relationship in the hostpathogen system Malusxrobusta 5Erwinia amylovora
Gene-for-gene relationship in the hostpathogen system Malusxrobusta 5Erwinia amylovora
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DOI：
10.1111/nph.12094
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发表时间：
2013-03-01
期刊：
影响因子：
9.4
通讯作者：
Peil, Andreas
中科院分区：
文献类型：
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作者：
Vogt, Isabelle;Woehner, Thomas;Peil, Andreas
火疫病是一种由火疫病菌引起的破坏性细菌性疾病，影响蔷薇科植物，包括苹果。宿主对火疫病的抗性主要存在于苹果属的种质中。并被认为在该病理系统中是定量的。在本研究中，我们分析了梨火疫病菌效应子 avrRpt2EA（丁香假单胞菌 avrRpt2 的同源物）对于 Malusxrobusta 5 (Mr5) 抗性的重要性。缺失突变体E.amylovora Ea1189avrRpt2EA能够克服Mr5的火疫病抗性。在几种对 Mr5 毒力不同的欧文氏菌菌株的 avrRpt2EA 中检测到一种单核苷酸多态性 (SNP)，导致编码蛋白中半胱氨酸变为丝氨酸。编码丝氨酸（S 等位基因）的梨火霉菌株能够克服 Mr5 的抗性，而编码半胱氨酸（C 等位基因）的菌株则不能。在本塞姆氏烟草中与拟南芥 RIN4 的共表达测定中也观察到等位基因特异性。在 Mr5 中检测到并分离出 RIN4 的同源物。这些结果表明，系统类似于拟南芥的 RPS2 和丁香假单胞菌的 AvrRpt2 与 RIN4 作为保护的相互作用。我们的数据表明宿主病原体系统 Mr5 和梨火疫病菌之间存在基因间关系。
Fire blight is a destructive bacterial disease caused by Erwinia amylovora affecting plants in the family Rosaceae, including apple. Host resistance to fire blight is present mainly in accessions of Malus spp. and is thought to be quantitative in this pathosystem. In this study we analyzed the importance of the E.amylovora effector avrRpt2EA, a homolog of Pseudomonas syringae avrRpt2, for resistance of Malusxrobusta 5 (Mr5). The deletion mutant E.amylovora Ea1189avrRpt2EA was able to overcome the fire blight resistance of Mr5. One single nucleotide polymorphism (SNP), resulting in an exchange of cysteine to serine in the encoded protein, was detected in avrRpt2EA of several Erwinia strains differing in virulence to Mr5. E.amylovora strains encoding serine (S-allele) were able to overcome resistance of Mr5, whereas strains encoding cysteine (C-allele) were not. Allele specificity was also observed in a coexpression assay with Arabidopsis thaliana RIN4 in Nicotiana benthamiana. A homolog of RIN4 has been detected and isolated in Mr5. These results suggest a system similar to the interaction of RPS2 from A.thaliana and AvrRpt2 from P.syringae with RIN4 as guard. Our data are suggestive of a gene-for-gene relationship for the hostpathogen system Mr5 and E.amylovora.