High-fat diet impairs the effects of a single bout of endurance exercise on glucose transport and insulin sensitivity in rat skeletal muscle
High-fat diet impairs the effects of a single bout of endurance exercise on glucose transport and insulin sensitivity in rat skeletal muscle
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DOI：
10.1016/j.metabol.2007.07.017
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发表时间：
2007-12-01
影响因子：
9.8
通讯作者：
Nakao, Kazuwa
中科院分区：
文献类型：
--
作者：
Tanaka, Satsuki;Hayashi, Tatsuya;Nakao, Kazuwa
单次运动通过胰岛素依赖和胰岛素依赖两种机制增加肌肉葡萄糖转运(GT)的速率。本研究的目的是确定高脂饮食(HFD)喂养是否干扰中等强度耐力运动诱导的代谢激活。大鼠喂饲高脂饲料或对照饲料(CD)4周后，在跑台上运动1h(19m/min，15%坡度)。两组运动后即刻解剖的比目鱼肌的胰岛素非依赖性GT明显高于久坐不动的大鼠肌肉，但高脂饲料喂养的大鼠比镉喂养的大鼠的胰岛素非依赖性GT低25%。在运动后2小时的肌肉解剖中，在存在亚最大有效浓度胰岛素(0.9nmol/L)的情况下，两组饮食组的胰岛素依赖性GT也较高，但高脂饲料喂养的大鼠比镉喂养的大鼠低25%。在HFD组，运动诱导的5‘-腺苷一磷酸活化蛋白激酶的激活相应地被钝化，5’-腺苷一磷酸激活蛋白激酶是导致胰岛素非依赖性GT和调节胰岛素敏感性的信号媒介。高脂饮食不影响葡萄糖转运蛋白4含量，也不影响胰岛素刺激的Akt磷酸化。我们的发现提供了证据，表明高脂饮食损害了短期耐力运动对葡萄糖代谢的影响，运动不能完全补偿高脂饮食引起的骨骼肌胰岛素抵抗。尽管潜在的机制尚不清楚，但运动过程中5‘-单磷酸腺苷活化蛋白激酶活性降低可能起到一定作用。(C)2007 Elsevier Inc.保留所有权利。
A single bout of exercise increases the rate of muscle glucose transport (GT) by both insulin-independent and insulin-dependent mechanisms. The purpose of this study was to determine whether high-fat diet (HFD) feeding interferes with the metabolic activation induced by moderate-intensity endurance exercise. Rats were fed an HFD or control diet (CD) for 4 weeks and then exercised on a treadmill for 1 hour (19 m/min, 15% incline). Insulin-independent GT was markedly higher in soleus muscle dissected immediately after exercise than in muscle dissected from sedentary rats in both dietary groups, but insulin-independent GT was 25% lower in HFD-fed than in CD-fed rats. Insulin-dependent GT in the presence of submaximally effective concentration of insulin (0.9 nmol/L) was also higher in both dietary groups in muscle dissected 2 hours after exercise, but was 25% lower in HFD-fed than in CD-fed rats. Exercise-induced activation of 5' adenosine monophosphate-activated protein kinase, a signaling intermediary leading to insulin-independent GT and regulating insulin sensitivity, was correspondingly blunted in the HFD group. High-fat diet did not affect glucose transporter 4 content or insulin-stimulated Akt phosphorylation. Our findings provide evidence that an HFD impairs the effects of short-term endurance exercise on glucose metabolism and that exercise does not fully compensate for HFD-induced insulin resistance in skeletal muscle. Although the underlying mechanism is unclear, reduced 5' adenosine monophosphate-activated protein kinase activation during exercise may play a role. (c) 2007 Elsevier Inc. All rights reserved.