Changes in redox state of albumin before and after kidney transplantation in patients with end-stage renal disease
Changes in redox state of albumin before and after kidney transplantation in patients with end-stage renal disease
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终末期肾病患者肾移植前后白蛋白氧化还原状态的变化
DOI：
10.1016/j.clinbiochem.2020.04.010
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发表时间：
2020
期刊：
影响因子：
2.8
通讯作者：
Hiroki Itoh
中科院分区：
文献类型：
--
作者：
Ryota Tanaka;Tadashi Imafuku;Yosuke Suzuki;Kento Nishida;Kotaro Matsusaka;Toshitaka Shin;Yuhki Sato;Yu Ishima;Hiroshi Watanabe;Hiromitsu Mimata;Toru Maruyama;Hiroki Itoh
目的心血管疾病是终末期肾病患者肾移植后的主要死亡原因之一。由于慢性肾脏病患者的心血管并发症与氧化应激密切相关，因此了解肾移植后的氧化应激状况对于预防心血管疾病具有重要意义。本研究旨在探讨肾移植后肾功能的改善是否对白蛋白的Cys 34残基的氧化还原状态有影响，反映了血液中的氧化应激水平。Design & MethodsWe招募了23例终末期肾功能衰竭患者接受肾移植。所有患者在移植后随访180天。白蛋白异构体的馏分进行了测定的电喷雾电离飞行时间质谱（ESI-TOFMS）method.ResultsSerum肌酐显着下降后立即肾移植，表明成功的移植。ESI-TOFMS方法鉴定了三种在Cys 34残基处半胱氨酸化的白蛋白亚型（Cys-Cys 34-白蛋白）和三种相应的无Cys 34半胱氨酸化的白蛋白亚型。总Cys-Cys 34-白蛋白的分数在肾移植后短暂降低，并且随后在第7天升高，此后逐渐降低直到第180天。同时，降低的白蛋白浓度直到肾移植后第14天才发生变化，然后在第30天与移植前水平相比显着增加，并保持稳定升高直至第180天。结论发现实际降低的白蛋白水平在肾移植后第30天或之后超过移植前水平，这与肾功能立即恢复不同。肾移植后肾功能立即恢复，但减少的白蛋白浓度增加高于移植前的水平，仅从移植后30天。
ObjectivesCardiovascular disease is one of the major causes of death in patients with end-stage kidney disease who have undergone kidney transplantation. Since the complication of cardiovascular disease in patients with chronic kidney disease is strongly linked to oxidative stress, understanding the oxidative stress condition after kidney transplantation would be of great importance for the prevention of cardiovascular disease. This study examined whether improvement of renal function after kidney transplantation has an impact on the redox state of the Cys34 residue of albumin that reflects the level of oxidative stress in blood.Design & methodsWe enrolled 23 patients with end-stage renal failure who received kidney transplantation. All patients were followed for 180 days after transplantation. The fractions of albumin isoforms were determined by the electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) method.ResultsSerum creatinine decreased significantly immediately after kidney transplantation, suggesting successful transplantations. The ESI-TOFMS method identified three albumin isoforms cysteinylated at the Cys34 residue (Cys-Cys34-albumin) and the three corresponding albumin isoforms without Cys34 cysteinylation. The fraction of total Cys-Cys34-albumin decreased transiently after kidney transplantation, and was followed by an elevation at day 7 and gradual decrease thereafter until day 180. Meanwhile, reduced albumin concentration did not change until day 14 after kidney transplantation, then showed a significant increase compared to pre-transplant level at day 30 and remained stably elevated until day 180.ConclusionsActual reduced albumin levels were found to exceed pre-transplant levels on or after day 30 following kidney transplantation unlike immediate restoration of renal function. Renal function was recovered immediately following kidney transplantation, but reduced albumen concentration increased above the pre-transplant levels only from day 30 after transplantation.