Chromosomal imbalances and DNA amplifications in SV40 large T antigen-induced primitive neuroectodermal tumor cell lines of the rat
Chromosomal imbalances and DNA amplifications in SV40 large T antigen-induced primitive neuroectodermal tumor cell lines of the rat
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DOI：
10.1093/carcin/20.8.1433
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发表时间：
1999-08-01
期刊：
影响因子：
4.7
通讯作者：
Scherthan, H
中科院分区：
文献类型：
--
作者：
Kappler, R;Pietsch, T;Scherthan, H
比较基因组原位杂交分析的四个细胞系来自SV 40大T抗原诱导的原始神经外胚层肿瘤的大鼠显示非复发性染色体拷贝数的变化和DNA扩增的染色体带2 q34，4 q43 qter和15 q12 qter的细胞系TZ 102，TZ 103和TZ 107，分别。半定量PCR和western blot分析显示，TZ 102细胞中N-ras原癌基因扩增并过表达，流式细胞术和细胞遗传学检测显示，所有细胞系均显示非整倍体细胞群和染色体数目可变。这些结果表明，DNA扩增以及基因组的不稳定性可能有助于SV 40大T抗原诱导的大鼠原始神经外胚层肿瘤的发病机制。
Comparative genomic in situ hybridization analysis of four cell lines derived from SV40 large T antigen-induced primitive neuroectodermal tumors of the rat revealed nonrecurrent chromosomal copy number changes and DNA amplifications at chromosomal bands 2q34, 4q43qter and 15q12qter in cell lines TZ102, TZ103 and TZ107, respectively. Semi-quantitative PCR and western blot analysis demonstrated amplification and over-expression of the rat N-ras proto-oncogene in TZ102, Furthermore, all cell lines displayed aneuploid cell populations and variable chromosome numbers as assessed by flow cytometry and cytogenetics. These findings suggest that DNA amplification as well as genomic instability may contribute to the pathogenesis of SV40 large T antigen-induced primitive neuroectodermal tumors of the rat.