Evolution and organization of the human protein C gene.
Evolution and organization of the human protein C gene.
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人类蛋白 C 基因的进化和组织。
DOI：
10.1073/pnas.83.3.546
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发表时间：
1986
影响因子：
11.1
通讯作者：
Crabtree,GR
中科院分区：
文献类型：
--
作者：
Plutzky,J;Hoskins,JA;Long,GL;Crabtree,GR
我们已经分离出重叠的噬菌体基因组克隆，覆盖了编码人类蛋白C基因的21种酶的区域。该基因至少有11.2个碱基长，由9个外显子和8个内含子组成。与表皮生长因子和转化生长因子同源的两个区域由氨基酸46-91和92-136编码，并由内含子精确界定，凝血因子IX和组织纤溶酶原激活物基因中的类似序列也是如此。当因子IX和蛋白C的同源氨基酸对齐时，所有八个内含子的位置精确地对应，表明这些基因是相对较新的基因复制的产物。然而，这两个基因是足够远的相关，没有核酸同源性保留在内含子区域，内含子的大小在两个基因之间变化显着。因子IX和蛋白C基因的相似性表明它们可能是参与凝血和纤溶的丝氨酸蛋白酶基因家族中最密切相关的成员。
We have isolated overlapping phage genomic clones covering an area of 21 kilobases that encodes the human protein C gene. The gene is at least 11.2 kilobases long and is made up of nine exons and eight introns. Two regions homologous to epidermal growth factor and transforming growth factor are encoded by amino acids 46-91 and 92-136 and are precisely delimited by introns, as is a similar sequence in the genes for coagulation factor IX and tissue plasminogen activator. When homologous amino acids of factor IX and protein C are aligned, the positions of all eight introns correspond precisely, suggesting that these genes are the product of a relatively recent gene duplication. Nevertheless, the two genes are sufficiently distantly related that no nucleic acid homology remains in the intronic regions and that the size of the introns varies dramatically between the two genes. The similarity of the genes for factor IX and protein C suggests that they may be the most closely related members of the serine protease gene family involved in coagulation and fibrinolysis.