Structure-process-functional property relationship of nanostructured carbon mediated cellular response for soft-tissue reconstruction and replacement
Structure-process-functional property relationship of nanostructured carbon mediated cellular response for soft-tissue reconstruction and replacement
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DOI：
10.1016/j.actbio.2012.01.027
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发表时间：
2012-05-01
影响因子：
9.7
通讯作者：
Shah, J. S.
中科院分区：
文献类型：
--
作者：
Misra, R. D. K.;Depan, D.;Shah, J. S.
柔性和延展性硅胶被广泛用作软组织替代物，用于关节重建和置换，包括由先天性或后天因素（如骨关节炎）引起的关节疼痛和活动性丧失。虽然这些人工装置的预期寿命为15年或更长，但它们可能过早碎裂。这种失效的原因是抗拉强度低和器械周围骨形成不足，导致器械不与周围组织结合。因此，与骨接触的材料的持续挑战是设计具有调节细胞-基质相互作用以促进骨整合和长期稳定性的能力的高断裂强度硅胶。为此，我们已经发现了令人兴奋的证据，即在硅链之间的空隙空间中引入新型纳米结构的碳，结合在高压下的处理，有利于刺激细胞功能，并提供高度的细胞相容性。此外，保持了有机硅的高断裂强度和不降低的固有伸长率。为此，我们联合收割机在这里结合材料科学与工程和细胞生物学，阐明细胞-基质相互作用的机制和控制细胞反应的分子机制。这是通过研究细胞附着，增殖和形态学，包括细胞形态学评价和定量评估的突出蛋白质，肌动蛋白，黏着斑蛋白和纤连蛋白是敏感的细胞基质相互作用。该研究为利用纳米结构生物材料的纳米或量子尺寸效应奠定了基础。(C)2012 Acts Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The flexible and ductile silicone is widely used as a soft-tissue substitute for joint reconstruction and replacement in situations including joint pain and loss of mobility caused by the congenital or acquired factors such as osteoarthritis. Although these artificial devices have an expected life span of 15 years or more, they can fragment prematurely. Explanations for such failure are low tensile strength and inadequate bone build-up around the device, as a result of which the device does not bind with the surrounding tissues. Thus, the continued challenge for materials in contact with the bone is the design of high-strength-at-break silicone with the ability to modulate cell-substrate interactions for promoting osseointegration and long-term stability. To this end, we have discovered exciting evidence that the introduction of a novel nanostructured carbon in the void space between the silicone chains combined with processing at elevated pressure favorably stimulate cellular functions and provide a high degree of cytocompatibility. Furthermore, the high strength-at-break and undiminished intrinsic elongation of silicone are retained. In this regard, we combine here materials science and engineering and cellular biology, to elucidate the mechanism of cell-substrate interactions and the molecular machinery controlling the cell response. This is accomplished by investigating cell attachment, proliferation, and morphology, including cytomorphometric evaluation and quantitative assessment of prominent proteins, actin, vinculin, and fibronectin that are sensitive to cell-substrate interactions. The study strengthens the foundation for utilizing the nano- or quantum-size effects of nanostructured biomaterials. (C) 2012 Acts Materialia Inc. Published by Elsevier Ltd. All rights reserved.