Bursaphelenchus niphades n. sp (Tylenchina: Aphelenchoididae) amensally associated with Niphades variegatus (Roelofs) (Coleoptera: Curculionidae)
Bursaphelenchus niphades n. sp (Tylenchina: Aphelenchoididae) amensally associated with Niphades variegatus (Roelofs) (Coleoptera: Curculionidae)
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DOI：
10.1163/15685411-00002763
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发表时间：
2014-01-01
期刊：
影响因子：
1.2
通讯作者：
Kanzaki, Natsumi
中科院分区：
文献类型：
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作者：
Tanaka, Suguru E.;Tanaka, Ryusei;Kanzaki, Natsumi
从日本本土的蛀木象甲 Niphades variegatus 和枯死的松科树中分离出伞滑刃属 (Bursaphelenchus) 物种。线虫与象鼻虫作为 dauer（扩散第三阶段）幼虫相关，并且 dauer 进入象鼻气管系统，在象鼻气管（心房）上形成异常扩张。因此，线虫被假设为象鼻虫的非泳道/泳动伙伴，因为异常扩张似乎抑制了象鼻虫的呼吸。线虫的繁殖阶段与死树（木材和树皮材料）有关，并且被认为以自然繁殖的真菌为食。从形态上看，这个新物种被认为是与 B. Antoniae、B. Chengi 和 B. hylobianum 接近的未描述物种。在这四个物种中，新物种在本文中被描述为 B. niphades n。 sp.，与 B. hengi 最接近，即这两个物种的类型特征几乎彼此相同，并通过一些次要特征（雄性 P4 生殖乳头的结构和骨针长度）进行区分。分子系统发育分析支持形态学观察。 Niphades 伞滑刃线虫sp。与这四个物种形成了一个支持良好的分支，介于 B. hylobianum 和 B. Chengi 之间；然而，它通过一些核糖体RNA基因的分子序列来区分。由于这四个物种中的三个与象鼻虫物种相关，因此该分支被认为是“象鼻虫相关”物种组。
A Bursaphelenchus species was isolated from a Japanese native wood-boring weevil, Niphades variegatus, and dead Pinaceae trees. The nematode is associated with the weevils as dauer (dispersal third stage) juveniles and the dauers enter the weevil tracheal system forming an abnormal expansion on the weevil trachea (atrium). Thus, the nematode is hypothesised to be an amensal/phoretic associate of the weevil because the abnormal expansion appeared to inhibit weevil respiration. The propagative stages of the nematode are associated with dead trees (wood and bark materials) and are thought to feed there on naturally propagated fungi. Morphologically, the new species is considered an undescribed species close to B. antoniae, B. chengi and B. hylobianum. Within these four species, the new species, which is described herein as B. niphades n. sp., is closest to B. chengi, i.e., the typological character of these two species are almost identical to each other and is distinguished by some minor characters (structure of the male P4 genital papillae and spicule length). The molecular phylogenetic analysis supported the morphological observations. Bursaphelenchus niphades n. sp. formed a well supported subclade with the four species and is intermediate between B. hylobianum and B. chengi; however, it is distinguished by the molecular sequences of some ribosomal RNA genes. Because three of these four species are associated with weevil species, the subclade is considered a 'weevil-associated' species group.