Deciphering Phosphotyrosine-Dependent Signaling Networks in Cancer by SH2 Profiling.
Deciphering Phosphotyrosine-Dependent Signaling Networks in Cancer by SH2 Profiling.
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DOI：
10.1177/1947601912459048
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发表时间：
2012-05-01
期刊：
影响因子：
--
通讯作者：
Nollau, Peter
中科院分区：
文献类型：
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作者：
Machida, Kazuya;Khenkhar, Malik;Nollau, Peter
SH2图谱是一种模块化的基于区域的分子诊断工具，自推出以来已经有十年的时间了。本文综述了SH2图谱的最初概念、不同的分析平台及其应用，从单个蛋白质的详细分析到在翻译研究中的广泛筛选。通过实例，我们讨论了该方法的独特性和优势，以及它的局限性和挑战。我们为基础研究人员和肿瘤学家提供指导，他们可能会在各自的癌症研究中考虑SH2图谱，特别是对那些专注于酪氨酸磷酸蛋白质组学的人。Sh2图谱可以作为一种替代的磷酸蛋白质组学工具来剖析导致单个恶性肿瘤的异常酪氨酸激酶途径，目的是促进癌症治疗的个性化诊断。
It has been a decade since the introduction of SH2 profiling, a modular domain-based molecular diagnostics tool. This review covers the original concept of SH2 profiling, different analytical platforms, and their applications, from the detailed analysis of single proteins to broad screening in translational research. Illustrated by practical examples, we discuss the uniqueness and advantages of the approach as well as its limitations and challenges. We provide guidance for basic researchers and oncologists who may consider SH2 profiling in their respective cancer research, especially for those focusing on tyrosine phosphoproteomics. SH2 profiling can serve as an alternative phosphoproteomics tool to dissect aberrant tyrosine kinase pathways responsible for individual malignancies, with the goal of facilitating personalized diagnostics for the treatment of cancer.