Chip-based nanovials for tryptic digest and capillary electrophoresis
Chip-based nanovials for tryptic digest and capillary electrophoresis
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DOI：
10.1016/s0003-2670(99)00486-9
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发表时间：
1999-11-29
影响因子：
6.2
通讯作者：
Roeraade, J
中科院分区：
文献类型：
--
作者：
Litborn, E;Emmer, Å;Roeraade, J
已经开发了在基于芯片的开口小瓶中进行胰蛋白酶消化的方法，所述开口小瓶具有15 nl的体积。在具有增加的湿度的封闭环境中工作抵消了反应期间水从小瓶中的蒸发。天然肌红蛋白的肽图，获得了毛细管电泳和比较的结果，在传统的100 μ l的体积进行的电泳。在常规塑料小瓶和基于芯片的小瓶中进行反应后，肽图谱显示出相似的模式。与在常规塑料小瓶中进行的PCR相比，在基于芯片的小瓶中的反应显示出更有效。提高的效率可能是由于表面体积比和表面依赖反应的大的差异。用于在基于芯片的小瓶中进行单次消化的肌红蛋白的最低量为12 ng。相应的胰蛋白酶用量为0.2 ng。(C)1999 Elsevier Science B.V.保留所有权利。
A method for performing tryptic digests in chip-based open vials with volumes of 15 nl has been developed. Working in an enclosed environment with increased humidity counteracted the evaporation of water from the vials during the reaction. Peptide maps of native myoglobin were obtained with capillary electrophoresis and compared with the results of digests performed in conventional 100-mu l volumes. The peptide maps, after reactions performed in conventional plastic vials and chip-based vials, showed similar patterns. The reaction showed to be more efficient in the chip-based vials compared to digests performed in conventional plastic vials. The increased efficiency was probably due to the large differences in surface-to-volume ratios and surface dependent reactions. The lowest amount of myoglobin utilized to perform one single digest in a chip-based vial was 12 ng. The corresponding amount of trypsin used was 0.2 ng. (C) 1999 Elsevier Science B.V. All rights reserved.