Using metabolomic approaches to explore chemical diversity in rice.
Using metabolomic approaches to explore chemical diversity in rice.
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DOI：
10.1093/mp/ssu125
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发表时间：
2014
期刊：
影响因子：
27.5
通讯作者：
M. Kusano;Zhigang Yang;Yozo Okazaki;Ryo Nakabayashi;A. Fukushima;K. Saito
中科院分区：
文献类型：
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作者：
M. Kusano;Zhigang Yang;Yozo Okazaki;Ryo Nakabayashi;A. Fukushima;K. Saito
稻米 (Oryza sativa) 是一种极好的资源，占世界人口总热量摄入的 25%，稻米植物可产生多种生物活性化合物。为了确定水稻中代谢物的数量及其化学多样性，利用质谱（MS）和/或核磁共振波谱等分析技术研究了栽培稻的代谢物组成，并建立了水稻代谢物数据库。本综述总结了通过气相色谱-质谱、液相色谱-质谱和毛细管电泳-质谱检测水稻代谢物的最新知识，包括糖、氨基酸和有机酸、芳香族化合物和植物激素。还介绍了水稻植物产生的极性和非极性代谢物的生物学特性和活性。介绍并讨论了通过代谢组学方法估计未知代谢物结构的挑战。最后，介绍了成功应用水稻代谢物分析的例子，通过结合代谢物数量性状位点分析来表征对提高其质量潜在关键的基因，并识别在非生物胁迫条件下生长的水稻中发挥关键作用的潜在代谢物生物标志物。
Rice (Oryza sativa) is an excellent resource, it comprises 25% of the total caloric intake of the world's population and rice plants yield many types of bioactive compounds. To determine the number of metabolites in rice and their chemical diversity, the metabolite composition of cultivated rice has been investigated with analytical techniques such as mass spectrometry (MS) and/or nuclear magnetic resonance spectroscopy and rice metabolite databases have been constructed. This review summarizes current knowledge on metabolites in rice including sugars, amino and organic acids, aromatic compounds, and phytohormones detected by gas chromatography-MS, liquid chromatography-MS, and capillary electrophoresis-MS. The biological properties and the activities of polar and non-polar metabolites produced by rice plants are also presented. Challenges in the estimation of the structure(s) of unknown metabolites by metabolomic approaches are introduced and discussed. Lastly, examples are presented of the successful application of metabolite profiling of rice to characterize gene(s) potentially critical for improving its quality by combining metabolite quantitative trait loci analysis, and to identify potential metabolite biomarkers that play a critical role when rice is grown under abiotic stress conditions.