Diffractive micro bar codes for encoding of biomolecules in multiplexed assays
Diffractive micro bar codes for encoding of biomolecules in multiplexed assays
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DOI：
10.1021/ac7018574
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发表时间：
2008-03-15
影响因子：
7.4
通讯作者：
Morgan, Hywel
中科院分区：
文献类型：
--
作者：
Broder, Graham R.;Ranasinghe, Rohan T.;Morgan, Hywel
含有微米大小的衍射条形码的微粒已用寡核苷酸和免疫球蛋白 G 进行修饰，以实现 DNA 杂交和免疫测定。条形码是使用化学功能商用环氧光刻胶 (SU-8) 的光刻技术制造的。当通过合适的接头连接时，固定化探针分子对分析物表现出高亲和力和快速反应动力学，从而可以检测 DNA 序列中的单核苷酸差异并进行多重免疫分析。
Microparticles incorporating micrometer-sized diffractive bar codes have been modified with oligonucleotides and immunoglobulin Gs to enable DNA hybridization and immunoassays. The bar codes are manufactured using photolithography of a chemically functional commercial epoxy photoresist (SU-8). When attached by suitable linkers, immobilized probe molecules exhibit high affinity for analytes and fast reaction kinetics, allowing detection of single nucleotide differences in DNA sequences and multiplexed immunoassays in