ANTIBODY-RESPONSE TO HUMAN CYTOMEGALOVIRUS GLYCOPROTEIN-GB AND GLYCOPROTEIN-GH AFTER NATURAL INFECTION IN HUMANS
ANTIBODY-RESPONSE TO HUMAN CYTOMEGALOVIRUS GLYCOPROTEIN-GB AND GLYCOPROTEIN-GH AFTER NATURAL INFECTION IN HUMANS
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DOI：
10.1093/infdis/164.5.835
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发表时间：
1991-11-01
影响因子：
6.4
通讯作者：
MERIGAN, TC
中科院分区：
文献类型：
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作者：
RASMUSSEN, L;MATKIN, C;MERIGAN, TC
用免疫沉淀法对稳定表达人巨细胞病毒gB(Rgb)和rGH(RGH)的CHO细胞株进行免疫沉淀，研究了人巨细胞病毒重组gB(RgB)和重组Gh(RGH)糖蛋白在免疫活性和免疫低下人群中的抗体。对于RGB前体的抗体存在于60%的有免疫能力的个体中。然而，在这些患者中有10%的人检测到RGH抗体。在3名有症状的CMV单核细胞增多症患者中，在恢复期同时检测到RGB和RGH抗体，但在另外3名血清转换为CMV且无症状的患者中检测到较小程度的抗体。心脏和心肺移植受者的RGB和RGH抗体在初次和复发的CMV感染中都被检测到，但强度与免疫能力强的人不同。经选择的大量免疫血清球蛋白预防性应用于骨髓移植受者，往往缺乏RGH抗体，但不缺乏RGB抗体。
Antibody to the recombinant gB (rgB) and recombinant gH (rgH) glycoproteins of human cytomegalovirus (CMV) was studied in immunocompetent and immunocompromised humans by immunoprecipitating [S-35]methionine-labeled CHO cell lines stably expressing rgB and rgH. Antibody to the rgB precursor was present in > 60% of immunocompetent individuals. However rgH antibody was detected in < 10% of these patients. Antibody to both the rgB and rgH was detected during convalescence in three immunocompetent individuals with symptomatic CMV mononucleosis but to a lesser extent in three others who seroconverted to CMV without symptoms. Antibody to rgB and rgH in heart and heart-and-lung transplant recipients was detected in both primary and recurrent CMV infections but not with the same intensity as in immunocompetent individuals. Selected lots of immune serum globulin, administered prophylactically to bone marrow transplant recipients, were frequently deficient in antibody to rgH but not to rgB.