Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system
Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system
复制标题
DOI：
10.1016/j.bbrc.2004.11.060
复制
发表时间：
2005-01-21
影响因子：
3.1
通讯作者：
Park, EY
中科院分区：
文献类型：
--
作者：
Motohashi, T;Shimojima, T;Park, EY
家蚕是大规模生产真核蛋白和重组杆状病毒的理想宿主。苜蓿银纹夜蛾核型多角体病毒（Autographa californica nuclear polyhedrosis virus，AcNPV）的杆状病毒系统已经建立并得到广泛应用。然而，AcNPV不具有感染家蚕的潜力。我们建立了第一个可直接用于家蚕蛋白表达的家蚕核型多角体病毒杆粒系统。利用该系统，不仅通过重组病毒感染家蚕，而且通过直接注射家蚕杆状病毒质粒DNA，成功地在家蚕幼虫和蛹中表达了绿色荧光蛋白。该方法可在家蚕体内快速生产蛋白质，生产时间可达10天，且无生物危害，为将来重组真核蛋白和杆状病毒的生产提供了有力的工具。(C)2004爱思唯尔公司All rights reserved.
Silkworm is one of the most attractive hosts for large-scale production of eukaryotic proteins as well as recombinant baculoviruses for gene transfer to mammalian cells. The bacmid system of Autographa californica nuclear polyhedrosis virus (AcNPV) has already been established and widely used. However, the AcNPV does not have a potential to infect silkworm. We developed the first practical Bombyx mori nuclear polyhedrosis virus bacmid system directly applicable for the protein expression of silkworm. By using this system, the green fluorescence protein was successfully expressed in silkworm larvae and pupae not only by infection of its recombinant virus but also by direct injection of its bacmid DNA. This method provides the rapid protein production in silkworm as long as 10 days, is free from biohazard, thus will be a powerful tool for the future production factory of recombinant eukaryotic proteins and baculoviruses. (C) 2004 Elsevier Inc. All rights reserved.