Anatomical and histological changes in the oviducts of Japanese quail, Coturnix japonica, after embryonic exposure to ethynyloestradiol
Anatomical and histological changes in the oviducts of Japanese quail, Coturnix japonica, after embryonic exposure to ethynyloestradiol
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DOI：
10.1530/rep.0.1210155
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发表时间：
2001-01-01
期刊：
影响因子：
3.8
通讯作者：
Brunström, B
中科院分区：
文献类型：
--
作者：
Berg, C;Holm, L;Brunström, B
雌性鸟类的正常输卵管发育需要雌激素，但早期过量接触雌激素会导致输卵管异常并损害产卵能力。在这项研究中，在卵内暴露于合成雌激素乙炔雌二醇对未成熟和成年雌性日本鹌鹑，日本鹌鹑，输卵管的解剖学和组织学的影响进行了研究。在孵育第3天，将乙基雌二醇（2或20 ng g（-1）鸡蛋）注射到蛋黄中后，观察到一系列异常。炔雌醇诱导未成熟雏鸡管腔上皮和管状腺的早熟分化。右侧输卵管保留发生在所有年龄段的研究，而某些其他影响并不明显，直到性成熟。在性成熟的鸟类中，用乙炔雌二醇处理后，左输卵管的大小减小，子宫（壳腺）中的管状大密度降低。子宫阴道交界处比对照组长，管状腺密度更高。炔雌醇处理组鸡的枕大肌上皮细胞较高。胚胎暴露于环境污染物炔雌醇可能会导致鹌鹑输卵管持续的结构畸形，这可能会损害生育能力。由于输卵管畸形是胚胎暴露于外源性雌激素的指示，它们可能用作野生鸟类种群中外源性雌激素暴露的生物标志物。
Oestrogen is needed for normal oviductal development in female birds, but excessive early exposure to oestrogen can cause oviductal abnormalities and impair egg-laying ability. In this study, the anatomical and histological effects of in ovo exposure to the synthetic oestrogen ethynyloestradiol on the oviducts of immature and adult female Japanese quail, Coturnix japonica, were investigated. A series of abnormalities was observed after injection of ethyloestradiol (2 or 20 ng g(-1) egg) into the yolk on day 3 of incubation. Ethynyloestradiol induced precocious differentiation of the luminal epithelium and tubular glands in immature chicks. Right-side oviduct retention occurred at all the ages studied, whereas certain other effects were not evident until sexual maturity. The left oviduct was reduced in size and tubular grand density in the uterus (shell gland) was reduced in sexually mature birds that had been treated with ethynyloestradiol. The utero-vaginal junction was longer than in control birds and had a higher tubular gland density. The epithelial cells in the magnum were taller in birds treated with ethynyloestradiol. Embryonic exposure to the environmental contaminant ethynyloestradiol may cause persisting structural malformations in oviducts of quails, which can impair fertility. As oviductal malformations are indicative of embryonic exposure to exogenous oestrogen they are potentially useful as biomarkers of xeno-oestrogen exposure in wild bird populations.