WIDESPREAD DISPERSION OF NEURONAL CLONES ACROSS FUNCTIONAL REGIONS OF THE CEREBRAL-CORTEX
WIDESPREAD DISPERSION OF NEURONAL CLONES ACROSS FUNCTIONAL REGIONS OF THE CEREBRAL-CORTEX
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DOI：
10.1126/science.1734520
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发表时间：
1992-01-24
期刊：
影响因子：
56.9
通讯作者：
CEPKO, CL
中科院分区：
文献类型：
--
作者：
WALSH, C;CEPKO, CL
哺乳动物大脑的大脑皮层在进化过程中迅速扩张，获得了结构上可区分的区域，专门用于不同的功能。在某些大脑区域，在胚胎发育期间发生了对细胞混合的地形限制。作为一种手段，实验研究是否发生这种限制在大鼠新皮层的功能分区的形成过程中，克隆相关的新皮层细胞标记逆转录病毒介导的转移的组织化学标记基因。克隆边界是通过用遗传上不同的病毒库感染发育中的大脑，并通过聚合酶链反应扩增单个病毒基因组来确定的。大脑皮层中许多克隆相关的神经元变得广泛分散在皮层的功能区域中。因此，皮质区的特化发生在神经发生之后。
The cerebral cortex of the mammalian brain has expanded rapidly during the course of evolution and acquired structurally distinguishable areas devoted to separate functions. In some brain regions, topographic restrictions to cell intermixing occur during embryonic development. As a means of examining experimentally whether such restrictions occur during formation of functional subdivisions in the rat neocortex, clonally related neocortical cells were marked by retroviral-mediated transfer of a histochemical marker gene. Clonal boundaries were determined by infection of the developing brain with a library of genetically distinct viruses and amplification of single viral genomes by the polymerase chain reaction. Many clonally related neurons in the cerebral cortex became widely dispersed across functional areas of the cortex. Specification of cortical areas therefore occurs after neurogenesis.