New surfaces for desorption electrospray ionization mass spectrometry: porous silicon and ultra-thin layer chromatography plates
New surfaces for desorption electrospray ionization mass spectrometry: porous silicon and ultra-thin layer chromatography plates
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DOI：
10.1002/rcm.2566
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发表时间：
2006-01-01
影响因子：
2
通讯作者：
Cooks, R. Graham
中科院分区：
文献类型：
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作者：
Kauppila, Tiina J.;Talaty, Nari;Cooks, R. Graham
将纳米多孔硅（pSi）和超薄层色谱（UTLC）板作为解吸电喷雾电离（DESI）表面的性能与聚甲基丙烯酸甲酯（PMMA）和聚四氟乙烯（PTFE）的性能进行了比较，这两种表面在以前的DESI研究中都是流行的。使用所有四种表面测定六种不同供试化合物的检测限（LOD）和其他分析特性。化合物的LOD在fmol-pmol（pg-ng）范围内。当在样品施加期间将热施加到表面时，对于每种化合物，具有pSi表面的LOD得到进一步改善，这给出了与用PMMA和PTFE实现的LOD一样低或更低的LOD。UTLC板被成功地用作快速色谱分离的手段，然后再进行质谱分析。使用较新的pSi和UTLC表面实现的另一个优点是与溶液相样品的干燥相关的分析速度增加。这在UTLC表面立即发生，并且可以通过轻轻加热pSi表面来快速实现。样品中盐的存在不会导致任何表面的分析物信号的抑制。版权所有（c）2006约翰威利父子有限公司。
The performance of nanoporous silicon (pSi) and ultra-thin layer chromatography (UTLC) plates as surfaces for desorption electrospray ionization (DESI) was compared with that of polymethyl methacrylate (PMMA) and polytetrafluoroethylene (PTFE), both popular surfaces in previous DESI studies. The limits of detection (LODs) and other analytical characteristics for six different test compounds were determined using all four surfaces. The LODs for the compounds were in the fmol-pmol (pg-ng) range. The LODs with the pSi surface were further improved for each of the compounds when heat was applied to the surface during sample application which gave LODs as low as or lower than those achieved with PMMA and PTFE. The UTLC plates were successfully used as a rapid means of chromatographic separation prior to DESI-MS analysis. Another advantage achieved using the newer pSi and UTLC surfaces was increased speed of analysis, associated with drying of solution-phase samples. This took place immediately at the UTLC surface and it could be achieved rapidly by gently heating the pSi surface. The presence of salts in the sample did not cause suppression of the analyte signal with any of the surfaces. Copyright (c) 2006 John Wiley & Sons, Ltd.