Expression of αv integrin family in gastric carcinomas:: Increased αvβ6 is associated with lymph node metastasis
Expression of αv integrin family in gastric carcinomas:: Increased αvβ6 is associated with lymph node metastasis
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DOI：
10.1078/0344-0338-00355
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发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Oda, Y
中科院分区：
文献类型：
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作者：
Kawashima, A;Tsugawa, S;Oda, Y
为了研究整合素在人胃癌组织中的表达变化，我们采用逆转录聚合酶链反应（RT-PCR）和竞争性RT-PCR分析了α - v亚基和5种β -亚基。胃癌组织中alphav、beta6和beta8的表达率明显高于非肿瘤胃粘膜组织（NGMTs）。RT-PCR结果显示，18例α v和β 6亚基共表达的肿瘤中，有17例（94%）出现淋巴结转移（p = 0.0033）。免疫组织化学证实了这种倾向；免疫组织化学检测到alphavbeta6整合素的大多数病例（23/ 28,82%）出现淋巴结转移（p = 0.0193）。RT-PCR和免疫组化研究显示，胃癌组织中均表达beta5亚基。此外，在使用竞争性RT-PCR的定量分析中，胃癌中beta5的平均表达水平约为NGMTs的140倍。大多数胃癌患者(27/38,71%)β 8亚基免疫组化阳性。这些发现提示，alphav整合素家族的一些成员（alphavP5、alphavbeta6、alphavbeta8）表达上调，而alphavbeta6整合素可能参与了胃癌的淋巴转移。
To investigate the alterations as to integrin expression in human gastric carcinomas, we analyzed the alphav subunit and 5 types of beta subunits using reverse transcription-polymerase chain reaction (RT-PCR) and competitive RT-PCR. The incidence of alphav, beta6 and beta8 expression was significantly higher in carcinoma tissues than in nonneoplastic gastric mucosal tissues (NGMTs). Out of 18 carcinoma cases with coexpression of alphav and beta6 subunits, which was demonstrated by RT-PCR, 17 cases (94%) showed lymph node metastasis (p = 0.0033). This tendency was confirmed by immunohistochemistry; most cases (23/28, 82%) in which alphavbeta6 integrin was immunohistochemically detected showed lymph node metastasis (p = 0.0193). RT-PCR and immunohistochemical studies showed that gastric carcinoma tissues expressed beta5 subunit in all cases. Furthermore, in a quantitative analysis using competitive RT-PCR, the mean level of beta5 expression was approximately 140 times higher in gastric carcinomas than in NGMTs. Most gastric carcinoma cases (27/38, 71%) were immunohistochemically positive for beta8 subunit. These findings suggest that some members of the alphav integrin family (alphavP5, alphavbeta6, alphavbeta8) are up-regulated, and that alphavbeta6 integrin may be involved in the lymphatic metastasis of gastric carcinomas.