Precipitation of an insoluble product on enzyme monolayer electrodes for biosensor applications: Characterization by faradaic impedance spectroscopy, cyclic voltammetry, and microgravimetric quartz crystal microbalance analyses
Precipitation of an insoluble product on enzyme monolayer electrodes for biosensor applications: Characterization by faradaic impedance spectroscopy, cyclic voltammetry, and microgravimetric quartz crystal microbalance analyses
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DOI：
10.1021/ac9901541
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发表时间：
1999-08-01
影响因子：
7.4
通讯作者：
Willner, I
中科院分区：
文献类型：
--
作者：
Patolsky, F;Zayats, M;Willner, I
在单层功能化电极上沉淀不溶性绝缘产物可以开发新电化学生物传感器。法拉第阻抗谱和循环伏安法用于探测电极表面不溶产物积累时导电载体处的电子转移电阻。类似地，使用微重量石英晶体微天平（QCM）分析来测定电极上沉淀物的形成。使用辣根过氧化物酶 (HRP) 单层电极通过 4-氯-1-萘酚 (1) 的生物催化氧化和不溶性产物 (2) 的沉淀来分析 H2O2。由 HRP 和葡萄糖氧化酶 GOx 组成的双酶层电极用于感测葡萄糖。在 GOx 存在下，O-2 对葡萄糖进行生物催化氧化，产生 H2O2，产生的过氧化氢在 HRP 存在下影响不溶性产物 (2) 的形成。不溶性产物在电极上积累，以及由此产生的电子转移电阻的程度，与H2O2或葡萄糖的量相关，并提取适当的校准曲线。
Precipitation of an insoluble, insulating product on monolayer-functionalized electrodes enables the development of neu electrochemical biosensors. Faradaic impedance spectroscopy and cyclic voltammetry are used to probe the electron transfer resistance at the conductive support upon the accumulation of the insoluble product on the electrode surface. Similarly, microgravimetric quartz crystal microbalance, QCM, analyses were used to assay the formation of the precipitate on the electrode. A horseradish peroxidase, HRP, monolayer electrode is used to analyze H2O2 via the biocatalyzed oxidation of 4-chloro-1-naphthol (1) and the precipitation of the insoluble product (2). A bienzyme-layered electrode consisting of HRP and glucose oxidase, GOx, is used to sense glucose. Biocatalyzed oxidation of glucose by O-2, in the presence of GOx, yields H2O2, and the generated hydrogen peroxide effects the formation of the insoluble product (2) in the presence of HRP. The insoluble product accumulated on the electrode, and the extent of the resulting electron-transfer resistance, correlated with the amounts of H2O2 or glucose, and appropriate calibration curves are extracted.