Cell aggregation by scaffolded receptor clusters
Cell aggregation by scaffolded receptor clusters
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DOI：
10.1016/s1074-5521(02)00102-3
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发表时间：
2002-02-01
影响因子：
--
通讯作者：
Kiessling, LL
中科院分区：
文献类型：
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作者：
Gestwicki, JE;Strong, LE;Kiessling, LL
凝集素或抗体引起的细胞聚集对于生物技术和治疗应用是重要的。增加这些介质的亲合力和聚集性质的一种策略是使其配体结合位点的数量最大化。然而，凝集素和抗体的化合价受到其四级结构的限制。为了克服这一局限性，我们探讨了使用开环易位聚合（ROMP）作为支架非共价组装多个拷贝的凝集素，四价蛋白伴刀豆球蛋白A（Con A）产生的聚合物。我们证明，Con A和多价支架之间的复合物聚集的T细胞白血病细胞系（Jurkat）比单独Con A更有效。我们预计，合成支架将提供一种新的手段，促进依赖于细胞聚集的过程，如病原体清除和免疫识别。
The aggregation of cells by lectins or antibodies is important for biotechnological and therapeutic applications. One strategy to augment the avidity and aggregating properties of these mediators is to maximize the number of their ligand binding sites. The valency of lectins and antibodies, however, is limited by their quaternary structures. To overcome this limitation, we explored the use of polymers generated by ring-opening metathesis polymerization (ROMP) as scaffolds to noncovalently assemble multiple copies of a lectin, the tetravalent protein concanavalin A (Con A). We demonstrate that complexes between Con A and multivalent scaffolds aggregate cells of a T cell leukemia line (Jurkat) more effectively than Con A alone. We anticipate that synthetic scaffolds will offer a new means of facilitating processes that rely on cell aggregation, such as pathogen clearance and immune recognition.