Enhanced Osteogenesis in Cocultures with Human Mesenchymal Stem Cells and Endothelial Cells on Polymeric Microfiber Scaffolds
Enhanced Osteogenesis in Cocultures with Human Mesenchymal Stem Cells and Endothelial Cells on Polymeric Microfiber Scaffolds
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DOI：
10.1089/ten.tea.2013.0256
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发表时间：
2013-12-01
影响因子：
4.1
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Gershovich, Julia G.;Dahlin, Rebecca L.;Mikos, Antonios G.
在这项工作中，人骨髓间充质干细胞（hMSCs）和他们的成骨预培养衍生物与人脐静脉内皮细胞（HUVEC）的静电纺丝三维聚（-己内酯）微纤维支架直接共培养，以评估共培养的成骨结构的产生的影响。具体而言，将细胞在支架上培养长达3周，并评估细胞构成、碱性磷酸酶（ALP）活性和骨样基质形成。具有共培养物和单培养物的构建体在第一周后具有几乎相同的细胞结构，然而，在随后的2周培养期间，与单培养物相比，在共培养物中观察到较低的细胞结构。支架与共培养物显示出显着更高的ALP活性，糖胺聚糖和胶原蛋白的生产，以及更大的钙沉积在研究过程中相比，单一培养的hMSCs。此外，在含有成骨预培养和非预培养hMSCs的共培养物中，成骨结果同样稳健。结果表明，MSC和HUVEC群体在成骨培养条件下在多孔支架材料内的组合是促进成骨的有效策略。
In this work, human mesenchymal stem cells (hMSCs) and their osteogenically precultured derivatives were directly cocultured with human umbilical vein endothelial cells (HUVECs) on electrospun three-dimensional poly(-caprolactone) microfiber scaffolds to evaluate the coculture's effect on the generation of osteogenic constructs. Specifically, cells were cultured on scaffolds for up to 3 weeks, and the cellularity, alkaline phosphatase (ALP) activity, and bone-like matrix formation were assessed. Constructs with cocultures and monocultures had almost identical cellularity after the first week, however, lower cellularity was observed in cocultures compared to monocultures during the subsequent 2 weeks of culture. Scaffolds with cocultures showed a significantly higher ALP activity, glycosaminoglycan and collagen production, as well as greater calcium deposition over the course of study compared to monocultures of hMSCs. Furthermore, the osteogenic outcome was equally robust in cocultures containing osteogenically precultured and non-precultured hMSCs. The results demonstrate that the combination of MSC and HUVEC populations within a porous scaffold material under osteogenic culture conditions is an effective strategy to promote osteogenesis.