Determining nuclear morphology using an improved angle-resolved low coherence interferometry system
Determining nuclear morphology using an improved angle-resolved low coherence interferometry system
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DOI：
10.1364/oe.11.003473
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发表时间：
2003-12-15
期刊：
影响因子：
3.8
通讯作者：
Wax, A
中科院分区：
文献类型：
--
作者：
Pyhtila, JW;Graf, RN;Wax, A
我们概述了使用深度分辨光散射测量确定亚表面上皮细胞核形态的过程。使用第二代角度分辨低相干干涉测量系统完成测量。与最初的原型系统相比，新系统大大缩短了数据采集和分析时间。新系统的校准证明在散射研究，以确定在混浊的样品中的聚苯乙烯微球的尺寸分布。确定细胞核大小的过程是通过分析完整的，未染色的上皮组织的次表层中的基底细胞的测量值来讨论的。(C)2003年美国光学学会。
We outline the process for determining the morphology of subsurface epithelial cell nuclei using depth- resolved light scattering measurements. The measurements are accomplished using a second generation angle- resolved low coherence interferometry system. The new system greatly improves data acquisition and analysis times compared to the initial prototype system. The calibration of the new system is demonstrated in scattering studies to determine the size distribution of polystyrene microspheres in a turbid sample. The process for determining the size of cell nuclei is discussed by analyzing measurements of basal cells in a sub- surface layer of intact, unstained epithelial tissue. (C) 2003 Optical Society of America.