Label-acquired magnetorotation for biosensing: An asynchronous rotation assay
Label-acquired magnetorotation for biosensing: An asynchronous rotation assay
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DOI：
10.1016/j.jmmm.2010.09.014
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发表时间：
2011-02-01
影响因子：
2.7
通讯作者：
Kopelman, Raoul
中科院分区：
文献类型：
--
作者：
Hecht, Ariel;Kinnunen, Paivo;Kopelman, Raoul
本文提出了一种新的应用磁性粒子的生物传感，称为标签获得磁旋转（LAM）。该方法是基于传统的夹心测定格式与异步磁珠旋转（AMBR）方法的组合。在标记获得的磁旋转中，分析物促进磁性标记珠与固相球的结合，形成夹心复合物。然后将夹心复合物置于旋转磁场中，其中夹心复合物的旋转频率是附着于球体表面的分析物的量的函数。在这里，我们使用链霉亲和素包被的珠子和生物素包被的颗粒作为分析物模拟物，在未来的工作中被蛋白质和其他生物靶标取代。我们表明，这种传感方法具有两个数量级的动态范围。(C)2010 Elsevier B.V.版权所有。
This paper presents a novel application of magnetic particles for biosensing, called label-acquired magnetorotation (LAM). This method is based on a combination of the traditional sandwich assay format with the asynchronous magnetic bead rotation (AMBR) method. In label-acquired magnetorotation, an analyte facilitates the binding of a magnetic label bead to a nonmagnetic solid phase sphere, forming a sandwich complex. The sandwich complex is then placed in a rotating magnetic field, where the rotational frequency of the sandwich complex is a function of the amount of analyte attached to the surface of the sphere. Here, we use streptavidin-coated beads and biotin-coated particles as analyte mimics, to be replaced by proteins and other biological targets in future work. We show this sensing method to have a dynamic range of two orders of magnitude. (C) 2010 Elsevier B.V. Allrightsreserved.