Agarose gel electrophoresis.
Agarose gel electrophoresis.
复制标题
DOI：
10.1002/0471142727.mb0205as51
复制
发表时间：
2001-05-01
影响因子：
--
通讯作者：
Voytas, D
中科院分区：
文献类型：
--
作者：
Voytas, D
琼脂糖凝胶电泳是一种简单且高效的分离、鉴定和纯化 0.5 至 25 kb DNA 片段的方法。该方案可分为三个阶段：（1）用适合待分离DNA片段大小的琼脂糖浓度制备凝胶； (2) 将 DNA 样品装入样品孔中，并在可实现最佳分离的电压和时间段下运行凝胶； (3) 对凝胶进行染色，或者如果已将溴化乙锭掺入凝胶和电泳缓冲液中，则在紫外光照射下直接观察。
Agarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5- to 25-kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) the DNA samples are loaded into the sample wells and the gel is run at a voltage and for a time period that will achieve optimal separation; and (3) the gel is stained or, if ethidium bromide has been incorporated into the gel and electrophoresis buffer, visualized directly upon illumination with UV light.