Immobilized metal affinity chromatography in the presence of arginine
Immobilized metal affinity chromatography in the presence of arginine
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DOI：
10.1016/j.bbrc.2009.01.054
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发表时间：
2009-04-10
影响因子：
3.1
通讯作者：
Tsumoto, Kouhei
中科院分区：
文献类型：
--
作者：
Abe, Ryota;Kudou, Motonori;Tsumoto, Kouhei
Arginine hydrochloride (ArgHCl) is a versatile solvent additive, as it suppresses protein aggregation. ArgHCl has been used for protein refolding and to solubilize proteins from loose inclusion bodies. Immobilized metal affinity chromatography (IMAC) is one of the most commonly used technologies for purification of recombinant proteins. Here we have evaluated compatibility of ArgHCl with IMAC purification for his-tag proteins. ArgHCl clearly interfered with protein binding to Ni-columns. Nevertheless, such interference was greatly reduced at ArgHCl concentration below 200 mM, demonstrating that IMAC purification can be done even in the presence of ArgHCl. (C) 2009 Elsevier Inc. All rights reserved.
