Monoclonal antibodies to cerebellar pinceau terminals obtained after immunization with brain mRNA-injected Xenopus oocytes.
Monoclonal antibodies to cerebellar pinceau terminals obtained after immunization with brain mRNA-injected Xenopus oocytes.
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用脑 mRNA 注射的非洲爪蟾卵母细胞免疫后获得的小脑青皮末梢的单克隆抗体。
DOI：
10.1073/pnas.87.2.528
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发表时间：
1990
影响因子：
11.1
通讯作者：
Miledi,R
中科院分区：
文献类型：
--
作者：
Tigyi,G;Matute,C;Miledi,R
建立了以非洲爪蟾卵母细胞为免疫载体制备抗脑细胞抗原单克隆抗体的方法。该方法包括向非洲爪蟾卵母细胞注射大鼠脑mRNA以表达外源蛋白，并使用卵母细胞进行免疫。免疫接种之前，免疫耐受小鼠天然卵母细胞膜抗原。通过这种方法，我们产生了一组单克隆抗体，这些抗体是小脑“pineau”的特异性标记物--一种在篮状细胞末端和浦肯野细胞轴突的起始段之间形成的独特的复杂突触。我们的研究结果揭示了免疫反应高度本地化的pinceau和它的后期表达出生后第19天开始在小脑发育。
A method was developed to produce monoclonal antibodies to brain cell antigens by using Xenopus oocytes as immunological vectors. The method consists in injecting Xenopus oocytes with rat brain mRNA to express foreign proteins and using the oocytes for immunization. Immunizations were preceded by immunotolerization of mice to antigens of native oocyte membranes. With this approach we generated a set of monoclonal antibodies that are specific markers for the cerebellar "pinceau"--a unique complex synapse formed between basket cell terminals and the initial segment of the Purkinje cell axon. Our findings reveal an immunoreactivity highly localized at the pinceau and its late expression beginning at postnatal day 19 during cerebellar development.