REPRODUCTIVE BIOLOGY OF PMSG-PRIMED IMMATURE FEMALE RATS
REPRODUCTIVE BIOLOGY OF PMSG-PRIMED IMMATURE FEMALE RATS
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DOI：
10.1095/biolreprod13.1.38
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发表时间：
1975-01-01
影响因子：
3.6
通讯作者：
MEYER, RK
中科院分区：
文献类型：
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作者：
NUTI, KM;SRIDHARAN, BN;MEYER, RK
研究了PMSG诱导的未成熟雌性大鼠(30日龄8IU PMSG)的繁殖性能，以确定它们是否适合作为生殖研究中成年动物的模型或替代品。阴道涂片白细胞涂片显示，第33天宫颈刺激诱导假孕持续12天。子宫创伤在假孕第5天引起蜕膜细胞反应，4天内创伤角的平均重量增加15倍。经PMSG处理的幼鼠可以受孕，维持存活的胎儿到足月，并在正常人的情况下产下存活的幼鼠(平均妊娠期：24.2天；平均产仔数：7.1只)。用放射免疫法测定妊娠6~21天间每隔3天采血一次的血清孕酮、20α-二氢孕酮(20α-DHP)、黄体生成素和卵泡刺激素水平。孕酮从第6天的86.7 ng/ml降至第9天的69.7 ng/ml，第12天升至111.4 ng/ml，第21天降至64.4 ng/ml。20α-DHP从第6天的7.2 ng/ml上升到第15天的峰值14.5 ng/ml，第18天显著下降至5.8 ng/ml，血清LU和FSH值变化不明显。在成年妊娠大鼠中，这些激素谱与其他人报道的相似。PMSG刺激的妊娠大鼠在第3天切除双侧卵巢并每天注射2毫克黄体酮，推迟了植入。在第10天注射1μ雌酮诱导着床。在诱导着床后第7天，平均每只动物有8.2个着床部位。经PMSG处理的幼鼠出生的第一窝和第二窝的生长曲线与未处理的成年大鼠出生的第一窝和第二窝没有什么不同。第一胎和第二胎都被维持到成年。当来自同一窝(即第一窝或第二窝)的雄性和雌性随机交配时，它们的繁殖性能与正常成年大鼠相当。
The reprodutive performance of PMSG-primed immature female rats (8 IU PMSG at 30 days of age) was studied to determine whether they are suitable models or substitutes for adult animals in studies of reproduction. Pseudopregnancy, induced by cervical stimulation on day 33, lasted 12 days as indicated by leukocytic vaginal smears. Decidual cell reaction was elicited by uterine trauma on day 5 of pseudopregnancy, and resulted in a fifteen-fold increase in the mean weight of the traumatized horn within 4 days.PMSG-treated immature rats can conceive, maintain viable fetuses to term, and deliver live pups in a normal mannner (mean length of gestation: 24.2 days; mean litter size: 7.1 pups). Serum levels of progesterone, 20α-dihydroprogesterone (20α-DHP), LH, and FSH were measured by radioimmunoassay in samples taken at 3-day intervals between days 6 and 21 of pregnancy. Progesterone decreased from 86.7 ng/ml on day 6 to 69.7 ng/ml on day 9, was elevated to 111.4 ng/ml on day 12, and decreased to 64.4 ng/ml on day 21. 20α-DHP increased from 7.2 ng/ml on day 6 to a peak of 14.5 ng/ml on day 15, and decreased significantly to 5.8 ng/ml on day 18. Serum LU and FSH did not vary significantly. These hormone profiles are similar to those reported by others in adult pregnant rats.Implantation was delayed in PMSG-primed pregnant rats by bilateral ovariectomy on day 3 and the injection of 2 mg progesterone daily. Implantation was induced by an injection of 1μg estrone on day 10. There was an average of 8.2 implantation sites per animal on day 15, seven days after the induction of implantation.The postpartum reproductive performance of PMSG-treated mothers and their offspring was also studied. The growth curves for first and second litters born to PMSG-primed immature rats were not different from litters born to untreated adult rats. Both first and second litters were maintained to adulthood. When males and females from the same litters (i.e., first or second) were mated at random, their breeding performance was comparable to normal adult rats.