Expression of the Electrophysiological System During Murine Embryonic Stem Cell Cardiac Differentiation
Expression of the Electrophysiological System During Murine Embryonic Stem Cell Cardiac Differentiation
复制标题
小鼠胚胎干细胞心脏分化过程中电生理系统的表达
DOI：
10.1159/000074541
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发表时间：
2003
影响因子：
--
通讯作者：
M. V. D. van der Heyden
中科院分区：
文献类型：
--
作者：
Marjan van Kerrebroeck;A. V. van Ginneken;Ingrid de Grijs;Nancy A M Mutsaers;T. Opthof;H. Jongsma;M. V. D. van der Heyden
背景：基于干细胞的替代疗法被设想为一种修复因心肌细胞损失而导致的心脏衰竭的方法。为了防止潜在的致命性心律失常，供体细胞的电生理组成应与受体组织相匹配。为了设计所需的电生理表型，首先应该澄清离子通道和间隙连接蛋白的潜在分子调控。方法：采用半定量RT-PCR方法建立小鼠ES细胞向心肌细胞分化过程中7个主要心脏离子通道a亚基和4个主要心脏离子通道b亚基的表达，采用免疫组化方法建立2个主要心脏缝隙连接蛋白的表达。结果：离子通道mRNA表达谱呈序贯上调。Connexin-40和-43在早期心肌细胞中表达较低，在随后的分化阶段表达率升高。结论：小鼠胚胎干细胞的心脏分化以心脏主要离子通道和连接蛋白的序贯上调为特征。
Background: Stem cell based replacement therapy is envisioned as a method to repair failing hearts suffering from cardiomyocyte loss. To prevent potentially lethal arrhythmias, the donor cellular electrophysiological make-up should match with the acceptor tissue. To engineer the desired electrophysiological phenotype, the underlying molecular regulation of ion channels and gap-junction proteins should be clarified first. Methods: We established the expression of seven main cardiac ion channel a-subunits and four b-subunits using semiquantitive RT-PCR and two major cardiac gap-junction proteins by immunohistochemistry during the differentiation process of murine ES cells into cardiomyocytes. Results: Ion channel mRNA expression profiles display sequential upregulation. Connexin-40 and -43 expression is low in early cardiomyocytes, increased expression rates were found in subsequent differentiation phases. Conclusion: Cardiac differentiation of mouse embryonic stem cells is characterized by a sequential upregulation of the main cardiac ion channels and connexins.
影响因子：
15.9
作者：
Klug, MG;Soonpaa, MH;Field, LJ
通讯作者：
Field, LJ
影响因子：
37.8
作者：
S. Raman;G. Cooke;P. Binkley
通讯作者：
S. Raman;G. Cooke;P. Binkley