MONOCLONAL-ANTIBODIES SPECIFIC FOR GLIAL FIBRILLARY ACIDIC (GFA) PROTEIN AND FOR EACH OF THE NEUROFILAMENT TRIPLET POLYPEPTIDES
MONOCLONAL-ANTIBODIES SPECIFIC FOR GLIAL FIBRILLARY ACIDIC (GFA) PROTEIN AND FOR EACH OF THE NEUROFILAMENT TRIPLET POLYPEPTIDES
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DOI：
10.1111/j.1432-0436.1984.tb01355.x
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发表时间：
1983-01-01
期刊：
影响因子：
2.9
通讯作者：
OSBORN, M
中科院分区：
文献类型：
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作者：
DEBUS, E;WEBER, K;OSBORN, M
以猪胶质细胞酸性蛋白（GFA）为抗原，制备了10株鼠源性GFA单克隆抗体。尽管所有抗体在蛋白质印迹技术中识别从脊髓纯化的GFA，但基于它们对分子的限定片段的反应性，它们可以被细分为至少3组。在组织和细胞系上进行的单克隆抗体的免疫荧光染色模式类似于针对GFA的常规多克隆抗体所报告的模式。特别是星形胶质细胞和Bergmann神经胶质细胞被强烈染色。此外，分离并表征了对200 kD [kDalton]、160 kD或68 kD神经丝三联体蛋白具有特异性的小鼠单克隆抗体。这些抗体对神经元细胞具有特异性，并支持用类似抗原亲和纯化的多克隆抗体得出的结论。单克隆抗体的组合似乎是表征神经系统不同细胞类型的有价值的工具。
A panel of 10 mouse monoclonal antibodies specific for glial fibrillary acidic protein (GFA) was isolated using porcine GFA as antigen. Although all antibodies recognize GFA purified from spinal cord in the western blot technique, they can be subdivided into at least 3 groups on the basis of their reactivity against defined fragments of the molecule. Immunofluorescence staining patterns with the monoclonal antibodies performed on tissues and cell lines resembled those reported with conventional polyclonal antibodies directed against GFA. In particular astrocytes and Bergmann glia are strongly stained. In addition mouse monoclonal antibodies specific for either the 200 kD [kDalton], 160 kD or the 68 kD neurofilament triplet protein were isolated and characterized. These antibodies are specific for neuronal cells and support conclusions made with similar antigen affinity-purified polyclonal antibodies. The combined set of monoclonal antibodies seems a valuable tool to characterize the different cell types of the nervous system.