Expression of RBMX After Spinal Cord Injury in Rats
Expression of RBMX After Spinal Cord Injury in Rats
复制标题
大鼠脊髓损伤后RBMX的表达
DOI：
10.1007/s12031-012-9914-2
复制
发表时间：
2013-02-01
影响因子：
3.1
通讯作者：
Cui, Zhiming
中科院分区：
文献类型：
--
作者：
Zhang, Jinlong;Li, Debao;Cui, Zhiming
RBMX（RNA-binding motif gene，X chromosome，RNA结合基序基因）是一种异质性核内核糖核蛋白，与剪接体结合，影响选择性剪接。编码RBMX的基因位于染色体Xq 26上，并且广泛表达。然而，其在脊髓损伤中的表达和功能尚不清楚。本研究建立了成年大鼠急性脊髓挫伤（SCI）模型，研究了脊髓中RBMX表达的动态变化。Western blot和免疫组化结果显示，正常脊髓中存在RBMX。脊髓损伤后1d达高峰，14 d降至基础水平。免疫荧光双标显示RBMX免疫反应阳性细胞主要分布于神经元和星形胶质细胞，而小胶质细胞未见。有趣的是，RBMX表达增加主要是在神经元和星形胶质细胞。此外，RBMX/增殖细胞核抗原（PCNA）和RBMX/活性caspase-3共定位在GFAP和NeuN分别检测。我们还检测了活性caspase-3、bcl-2、Bax和PCNA的表达谱，其变化与RBMX的表达谱相关。总之，这是第一次描述RBMX在脊髓损伤中的表达。我们的研究结果表明，RBMX可能在脊髓损伤后中枢神经系统的病理生理学中起着至关重要的作用。
RBMX (RNA-binding motif gene, X chromosome) is a heterogeneous nuclear ribonucleoprotein that associates with the spliceosome, binds RNA, and influences alternative splicing. The gene encoding RBMX is located on chromosome Xq26 and is ubiquitously expressed. However, its expression and function in spinal cord injury are still unclear. In this study, we performed an acute spinal cord contusion injury (SCI) model in adult rats and investigated the dynamic changes of RBMX expression in spinal cord. Western blot and immunohistochemistry analysis revealed that RBMX was present in normal spinal cord. It gradually increased, reached a peak at 1 day, and then declined to basal levels at 14 days after spinal cord injury. Double immunofluorescence staining showed that RBMX immunoreactivity was found in neurons and astrocytes, but not in microglia. Interestingly, RBMX expression was increased predominantly in neurons and astrocytes. Moreover, colocalization of RBMX/proliferating cell nuclear antigen (PCNA) and RBMX/active caspase-3 was detected in GFAP and NeuN, respectively. We also examined the expression profiles of active caspase-3, bcl-2, Bax, and PCNA, whose changes were correlated with the expression profiles of RBMX. In conclusion, this is the first description of RBMX expression in spinal cord injury. Our results suggested that RBMX might play crucial roles in CNS pathophysiology after SCI.