Layer-specific dilation of penetrating arteries induced by stimulation of the nucleus basalis of Meynert in the mouse frontal cortex
Layer-specific dilation of penetrating arteries induced by stimulation of the nucleus basalis of Meynert in the mouse frontal cortex
复制标题
DOI：
10.1038/jcbfm.2013.92
复制
发表时间：
2013-09-01
影响因子：
6.3
通讯作者：
Kanno, Iwao
中科院分区：
文献类型：
--
作者：
Hotta, Harumi;Masamoto, Kazuto;Kanno, Iwao
为了阐明激活Meynert基底核（NBM）增加大脑皮质血流量的机制，我们研究了麻醉小鼠在NBM刺激期间皮质实质动脉是否扩张。我们使用双光子显微镜测量单个穿通动脉的直径在不同的深度（类似于800 μ m，层I至V）的额叶皮层，并检查直径的变化在局灶性电刺激的NBM（0.5毫秒，30至50 μ A和50 Hz）和高碳酸血症（3%CO2吸入）。刺激NBM引起穿通动脉直径增加9%至13%的刺激前直径在整个皮层的不同层，除了在皮层表面和上部的第V层，其中穿通动脉的直径仅略有增加，在NBM刺激。高碳酸血症引起包括表面动脉在内的所有皮质层穿通动脉明显扩张。在NBM刺激开始后1秒内，上皮层的直径开始增加，随后在下层增加。我们的研究结果表明，激活的NBM扩张皮层穿动脉在特定的方式在幅度和潜伏期，推测有关的胆碱能神经末梢的密度从NBM。
To clarify mechanisms through which activation of the nucleus basalis of Meynert (NBM) increases cerebral cortical blood flow, we examined whether cortical parenchymal arteries dilate during NBM stimulation in anesthetized mice. We used two-photon microscopy to measure the diameter of single penetrating arteries at different depths (similar to 800 mu m, layers I to V) of the frontal cortex, and examined changes in the diameter during focal electrical stimulation of the NBM (0.5 ms at 30 to 50 mu A and 50 Hz) and hypercapnia (3% CO2 inhalation). Stimulation of the NBM caused diameter of penetrating arteries to increase by 9% to 13% of the prestimulus diameter throughout the different layers of the cortex, except at the cortical surface and upper part of layer V, where the diameter of penetrating arteries increased only slightly during NBM stimulation. Hypercapnia caused obvious dilation of the penetrating arteries in all cortical layers, including the surface arteries. The diameters began to increase within 1 second after the onset of NBM stimulation in the upper cortical layers, and later in lower layers. Our results indicate that activation of the NBM dilates cortical penetrating arteries in a layer-specific manner in magnitude and latency, presumably related to the density of cholinergic nerve terminals from the NBM.