Endothelial cell adenylate cyclase: activation by catecholamines and prostaglandin I2+.
Endothelial cell adenylate cyclase: activation by catecholamines and prostaglandin I2+.
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内皮细胞腺苷酸环化酶：由儿茶酚胺和前列腺素 I2 激活。
DOI：
10.1016/0006-291x(80)91362-5
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发表时间：
1980
影响因子：
3.1
通讯作者：
Handin,RI
中科院分区：
文献类型：
--
作者：
Schafer,AI;GimbroneJr,MA;Handin,RI
完整的血管内皮细胞与1 mM 3-异丁基-1-甲基黄嘌呤，异丙肾上腺素或PGI 2孵育后，环AMP水平分别增加4倍和3倍。β-肾上腺素能阻断剂普萘洛尔可选择性抑制异丙肾上腺素刺激的细胞裂解物腺苷酸环化酶活性。儿茶酚胺对腺苷酸环化酶的刺激作用强弱顺序为异丙肾上腺素>肾上腺素>去甲肾上腺素。前列腺素没有刺激腺苷酸环化酶活性的细胞裂解物中，即使在鸟嘌呤核苷酸的存在下，或以下的完整细胞的预孵育与三尖杉酯碱之前的冻融裂解。
Following incubation of intact vascular endothelial cells with 1 mM 3-isobutyl-1-methylxanthine, and isoproterenol or PGI2, cyclic AMP levels increased 4- and 3-fold, respectively. Isoproterenol-stimulated adenylate cyclase activity of cell lysates was selectively inhibited by the β-adrenergic blocking agent propranolol. Catecholamines stimulated adenylate cyclase with the potency series: isoproterenol > epinephrine > norepinephrine. Prostaglandin did not stimulate adenylate cyclase activity in cell lysates, even in the presence of guanine nucleotides or following preincubation of the intact cells with prostaglandins prior to freeze-thaw lysis.