ACETYLCHOLINE RECEPTOR-REACTIVE LYMPHOCYTES-T FROM HEALTHY-SUBJECTS AND MYASTHENIA-GRAVIS PATIENTS
ACETYLCHOLINE RECEPTOR-REACTIVE LYMPHOCYTES-T FROM HEALTHY-SUBJECTS AND MYASTHENIA-GRAVIS PATIENTS
复制标题
DOI：
10.1212/wnl.41.8.1270
复制
发表时间：
1991-08-01
期刊：
影响因子：
9.9
通讯作者：
NEWSOMDAVIS, J
中科院分区：
文献类型：
--
作者：
SOMMER, N;HARCOURT, GC;NEWSOMDAVIS, J
114例重症肌无力（MG）患者中有23例（20%）的外周血淋巴细胞对从电鱼Torpedo中纯化的天然乙酰胆碱受体（AChR）表现出阳性T细胞增殖反应，而25例健康或其他神经系统疾病对照中有2例（8%）。 反应似乎随季节波动。 长期的T细胞系和克隆可以很容易地从两个健康的反应者从MG病例中选择，并表现出相似的培养行为，CD 4+表型，和HLA II类限制。 来自对照的一个克隆与重组人AChR α链（r37- 429 A）和来自其序列的合成肽125-143（S-S）交叉反应。 这两种人类抗原刺激原发性增殖反应在实质上更高的频率（26至59%）比原生异种乙酰胆碱受体-在患者和对照组-表明，真正的自身反应性T细胞不可避免地删除在正常的T细胞的发展。
Peripheral blood lymphocytes from 23 of 114 (20%) myasthenia gravis (MG) patients showed positive T-cell proliferative responses to native acetylcholine receptor (AChR) purified from the electric fish Torpedo, compared with two of 25 (8%) healthy or other neurologic disease controls. Responsiveness appeared to fluctuate seasonally. Long-term T-cell lines and clones could be selected as readily from the two healthy responders as from the MG cases and showed similar culture behavior, CD4 + phenotype, and HLA class II restrictions. One clone from a control cross-reacted with recombinant human AChR alpha chain (r37-429A) and with the synthetic peptide 125-143(S-S) from its sequence. Both these human antigens stimulated primary proliferative responses at substantially higher frequencies (26 to 59%) than native xeno-AChR-in both patients and controls-demonstrating that truly autoreactive T cells are not inevitably deleted during normal T-cell development.