Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila.
Accessibility to Peptidoglycan Is Important for the Recognition of Gram-Positive Bacteria in Drosophila.
复制标题
肽聚糖的可及性对于果蝇中革兰氏阳性细菌的识别很重要。
DOI：
10.1016/j.celrep.2019.04.103
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Vaz F
中科院分区：
文献类型：
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作者：
Vaz F
在果蝇中，肽聚糖识别蛋白 (PGRP) SA 和 LC 在结构上分别区分具有赖氨酸 (Lys) 或二氨基庚二酸 (DAP) 酸的细菌肽聚糖，从而诱导不同的抗菌转录反应。在这里，我们发现细胞壁上 PG 的可及性在感染免疫中发挥着核心作用。当壁磷壁酸 (WTA) 从 S 中被基因去除时。 金黄色葡萄球菌（Lys型）和枯草芽孢杆菌（DAP型），因此增加了可及性，两种PGRP与任一细菌的结合都增加了。 PGRP-SA 和 -LC 双突变果蝇更容易受到两种 WTA-less 细菌的感染。此外，无 WTA 的细菌在 PGRP-SA/-LC 双突变体果蝇中生长得更好。最后，感染无 WTA 的细菌会消除下游抗菌转录的任何差异激活。我们的结果表明，细胞壁 PG 的可及性是 PGRP 介导的免疫的主要因素，并且可能是区分病原体类别的原因。
InDrosophila, it is thought that peptidoglycan recognition proteins (PGRPs) SA and LC structurally discriminate between bacterial peptidoglycans with lysine (Lys) or diaminopimelic (DAP) acid, respectively, thus inducing differential antimicrobial transcription response. Here, we find that accessibility to PG at the cell wall plays a central role in immunity to infection. When wall teichoic acids (WTAs) are genetically removed fromS. aureus(Lys type) andBacillus subtilis(DAP type), thus increasing accessibility, the binding of both PGRPs to either bacterium is increased. PGRP-SA and -LC double mutant flies are more susceptible to infection with both WTA-less bacteria. In addition, WTA-less bacteria grow better in PGRP-SA/-LC double mutant flies. Finally, infection with WTA-less bacteria abolishes any differential activation of downstream antimicrobial transcription. Our results indicate that accessibility to cell wall PG is a major factor in PGRP-mediated immunity and may be the cause for discrimination between classes of pathogens.