An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
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DOI：
10.1111/1750-3841.15359
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发表时间：
2020-08
影响因子：
3.9
通讯作者：
B. Tonyali;C. Sommers;O. Cerić;J. Smith;U. Yucel
中科院分区：
文献类型：
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作者：
B. Tonyali;C. Sommers;O. Cerić;J. Smith;U. Yucel
干甘薯（SP）经常进行辐照，以提高安全性和保质期。使用电子顺磁共振（EPR）光谱分析辐射衍生的自由基的形成。这些辐照特异性自由基可用于表征含有纤维素和糖的干燥植物性食品的辐照历史。在不同的样品位置（皮肤和肉），作为样品制备方法（研磨，筛分和造粒）的函数的信号特性（强度和峰形）进行了评价。使用基于曲线下面积的峰的双重积分方法定量信号强度。筛滤引起的。与未筛分样品相比，由于纤维素基自由基的损失，总信号强度降低50%。辐照SP的果肉表现出复杂的EPR谱，有纤维素自由基的多个卫星峰（333.5和338.8mT）和葡萄糖自由基的分裂峰（337.4mT），而皮肤谱则是纤维素自由基的特征谱。在这项研究中，我们证明了样品的组成和制备方法的影响，形成和分析的辐射特定的自由基的基础上EPR。实际应用：在过去十年左右的时间里，已经出现了与食用辐照宠物食品相关的健康问题。电子顺磁共振光谱可用于分析含有纤维素和糖的干产品的辐照历史，例如受欢迎的狗食干红薯，以确保产品在安全范围内辐照。这项工作表明，辐射特定的自由基的形成是受样品的位置（皮肤和肉）和水分含量。
Dried sweet potatoes (SPs) are often irradiated for improved safety and shelf life. Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy. These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars. The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing). The signal intensity was quantified using a double integration method of the peaks based on the area under the curve. The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals. The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals. In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR. PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products. Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits. This work demonstrates that the formation of irradiation-specific radicals is affected by the sample location (skin and flesh) and moisture content.