Molecular Characterization of Human Respiratory Syncytial Virus in the Philippines, 2012-2013.
Molecular Characterization of Human Respiratory Syncytial Virus in the Philippines, 2012-2013.
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DOI：
10.1371/journal.pone.0142192
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Oshitani H
中科院分区：
文献类型：
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作者：
Malasao R;Okamoto M;Chaimongkol N;Imamura T;Tohma K;Dapat I;Dapat C;Suzuki A;Saito M;Saito M;Tamaki R;Pedrera-Rico GA;Aniceto R;Quicho RF;Segubre-Mercado E;Lupisan S;Oshitani H
人呼吸道合胞病毒（HRSV）是全球婴儿和儿童急性下呼吸道感染的主要原因。2012年6月至2013年7月，我们在菲律宾的四个研究中心（包括比利兰、莱特、巴拉望和大马尼拉）对临床诊断为严重肺炎的婴儿和儿童进行了HRSV分子分析。采集鼻咽拭子，并使用实时聚合酶链反应（PCR）筛查HRSV。阳性标本经常规PCR检测，并对G基因第二高变区（2nd HVR）进行测序。在总共1，505份样本中，423份样本（28.1%）为HRSV阳性，其中305份（72.1%）和118份（27.9%）分别鉴定为HRSV-A和HRSV-B。在研究期间，确定了两种HRSV-A基因型，NA 1和ON 1。在G基因的第二HVR中具有72个核苷酸重复的新的ON 1基因型迅速增加，并最终在2013年成为优势基因型，其进化速率高于NA 1基因型。此外，在ON 1基因型中，我们发现在氨基酸位置274处的正选择（p<0.05）和G基因的第二HVR中的大量O-和N-糖基化。在HRSV-B中，BA 9是菲律宾流行的主要基因型。然而，发现了两例GB 2基因型的散发病例，这可能与其他亚洲菌株有共同的祖先。这些研究结果表明，HRSV是菲律宾儿童严重急性呼吸道感染的一个重要原因，并揭示了ON 1基因型的出现和随后的优势和GB 2基因型的零星检测。这两种基因型都是在菲律宾首次发现的。
Human respiratory syncytial virus (HRSV) is a major cause of acute lower respiratory tract infections in infants and children worldwide. We performed molecular analysis of HRSV among infants and children with clinical diagnosis of severe pneumonia in four study sites in the Philippines, including Biliran, Leyte, Palawan, and Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected and screened for HRSV using real-time polymerase chain reaction (PCR). Positive samples were tested by conventional PCR and sequenced for the second hypervariable region (2nd HVR) of the G gene. Among a total of 1,505 samples, 423 samples were positive for HRSV (28.1%), of which 305 (72.1%) and 118 (27.9%) were identified as HRSV-A and HRSV-B, respectively. Two genotypes of HRSV-A, NA1 and ON1, were identified during the study period. The novel ON1 genotype with a 72-nucleotide duplication in 2nd HVR of the G gene increased rapidly and finally became the predominant genotype in 2013 with an evolutionary rate higher than the NA1 genotype. Moreover, in the ON1 genotype, we found positive selection at amino acid position 274 (p<0.05) and massive O- and N-glycosylation in the 2nd HVR of the G gene. Among HRSV-B, BA9 was the predominant genotype circulating in the Philippines. However, two sporadic cases of GB2 genotype were found, which might share a common ancestor with other Asian strains. These findings suggest that HRSV is an important cause of severe acute respiratory infection among children in the Philippines and revealed the emergence and subsequent predominance of the ON1 genotype and the sporadic detection of the GB2 genotype. Both genotypes were detected for the first time in the Philippines.