Genes important for survival or reproduction in Varroa destructor identified by RNAi
Genes important for survival or reproduction in Varroa destructor identified by RNAi
复制标题
RNAi 鉴定对破坏瓦螨生存或繁殖重要的基因
DOI：
10.1111/1744-7917.12513
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发表时间：
2019-02-01
期刊：
影响因子：
4
通讯作者：
Xie, Xianbing
中科院分区：
文献类型：
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作者：
Huang, Zachary Y.;Bian, Guowu;Xie, Xianbing
瓦螨（Varroa destructor）是世界范围内对蜜蜂健康最严重的威胁。为了探索利用RNA干扰技术防治瓦螨的可能性，我们测定了敲除不同基因对瓦螨存活和繁殖的影响。合成6个候选基因（Da、Pros 26 S、RpL 8、RpL 11、RpP 0和RpS 13）的双链RNA（dsRNA），并分别注射到瓦螨体内，然后评估螨的存活和繁殖。注射Da（无女儿）和Pros 26 S（蛋白酶体26 S亚基腺苷三磷酸酶基因）的dsRNA导致螨存活率显著降低，注射后72小时（hpi）螨存活率分别为3.57% ± 1.94%和30.03% ± 11.43%。注射绿色荧光蛋白（GFP）-dsRNA的对照螨的存活率分别为81.95% ± 5.03%和82.36 ± 2.81%。注射其他四种基因（RpL 8、RpL 11、RpP 0和RpS 13）的dsRNA对存活率没有显著影响，这使我们能够评估它们对瓦螨繁殖的影响。与GFP-dsRNA对照相比，注射这四种基因中每种基因的dsRNA的螨每只螨的雌性后代数量显著减少。通过真实的-时间聚合酶链反应验证靶基因的敲除，其中两个基因对生殖很重要（RpL 8、RpL 11），一个基因对存活很重要（Pros 26 S）。总之，通过RNA干扰，我们已经发现了两个重要的螨生存基因和四个重要的螨繁殖基因。这些基因可作为狄斯瓦螨防治的靶基因。
The Varroa mite, (Varroa destructor), is the worst threat to honey bee health worldwide. To explore the possibility of using RNA interference to control this pest, we determined the effects of knocking down various genes on Varroa mite survival and reproduction. Double‐stranded RNA (dsRNA) of six candidate genes (Da, Pros26S, RpL8, RpL11, RpP0 and RpS13) were synthesized and each injected into Varroa mites, then mite survival and reproduction were assessed. Injection of dsRNA for Da (Daughterless) and Pros26S (Gene for proteasome 26S subunit adenosine triphosphatase) caused a significant reduction in mite survival, with 3.57% ± 1.94% and 30.03% ± 11.43% mites surviving at 72 h post‐injection (hpi), respectively. Control mites injected with green fluorescent protein (GFP)‐dsRNA showed survival rates of 81.95% ± 5.03% and 82.36 ± 2.81%, respectively. Injections of dsRNA for four other genes (RpL8, RpL11, RpP0 and RpS13) did not affect survival significantly, enabling us to assess their effect on Varroa mite reproduction. The number of female offspring per mite was significantly reduced for mites injected with dsRNA of each of these four genes compared to their GFP‐dsRNA controls. Knockdown of the target genes was verified by real‐time polymerase chain reaction for two genes important for reproduction (RpL8, RpL11) and one gene important for survival (Pros26S). In conclusion, through RNA interference, we have discovered two genes important for mite survival and four genes important for mite reproduction. These genes could be explored as possible targets for the control of Varroa destructor in the future.